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Prognostic significance associated with the number of compound mutations in epidermal growth factor receptor-mutant non-small cell lung cancer

2022; Medycyna Praktyczna; Linguagem: Inglês

10.20452/pamw.16240

1897-9483

Hiroaki Satoh, Yuika Sasatani, Kunihiko Miyazaki, Yoshiharu Sato, Nobuyuki Hizawa,

Gastric Cancer Management and Outcomes

RESEARCH LETTER Compound mutations in the EGFR gene 1 patients with a single compound mutation in addition to the main EGFR mutation with those with 2 or more compound mutations. Methods MaterialsWe analyzed specimens collected from patients with the EGFR gene mutation treated in 2 tertiary hospitals (University of Tsukuba Mito Medical Center and Ryugasaki Saiseikai Hospital) since April 2009.Among them, a total of 109 patients were histopathologically diagnosed with EGFR -mutated NSCLC (including 63 [13.2%] of 476 and 46 [13.5%] of 341 NSCLC patients in each hospital, respectively).Of these, 47 consecutive patients with sufficient tissue specimens to enable a genetic analysis were included in this study.The performance status (PS) was assessed based on the Eastern Cooperative Oncology Group criteria, and a score of 2 or more was defined as poor PS.Analysis of EGFR mutations EGFR mutations were examined using a nonoverlapping integrated read sequencing system (DNA Chip Research Inc., Tokyo, Japan). 7,8Briefly, DNA was extracted from slices of formalin -fixed paraffin -embedded (FFPE) tissue blocks obtained from patients using a Maxwell RSC DNA FFPE kit (Promega, Madison, Wisconsin, United States).Double--stranded DNA was quantified with the Qubit dsDNA HS Assay (Thermo Fisher Scientific, Waltham, Massachusetts, United States) on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).A total of 50 ng of DNA was fragmented by a Covaris focused -ultrasonicator (Covaris Inc., Woburn, Massachusetts, United States) following the manufacturer's instructions.