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Development of Monoclonal Antibody 281-mG 2a -f Against Golden Hamster Podoplanin

2022; Mary Ann Liebert, Inc.; Volume: 41; Issue: 6 Linguagem: Inglês

10.1089/mab.2021.0058

2167-9436

Ren Nanamiya, Hiroyuki Suzuki, Junko Takei, Guanjie Li, Nohara Goto, Hiroyuki Harada, Masaki Saito, Tomohiro Tanaka, Teizo Asano, Mika K. Kaneko, Yukinari Kato,

Lymphatic Disorders and Treatments

Golden (Syrian) hamster (Mesocricetus auratus) is a small animal model of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Pathological analyses of the tissues are required to understand the pathogenesis of SARS-CoV-2 and the evaluation of therapeutic modalities, including neutralizing monoclonal antibodies (mAbs). However, mAbs that recognize the golden hamster-derived antigens and distinguish specific cell types, such as the pneumocytes, are limited. Podoplanin (PDPN) is an essential marker of lung type I alveolar epithelial cells, kidney podocytes, and lymphatic endothelial cells. In this study, an anti-Chinese hamster (Cricetulus griseus) PDPN mAb PMab-281 (IgG3, kappa) was established using the Cell-Based Immunization and Screening (CBIS) method. A defucosylated mouse IgG2a version of PMab-281 (281-mG2a-f) was also developed. The 281-mG2a-f strongly recognized both the Chinese hamster and the golden hamster PDPN using flow cytometry and could detect lung type I alveolar epithelial cells, lymphatic endothelial cells, and Bowman's capsules in the kidney from the golden hamster using immunohistochemistry. These results suggest the usefulness of 281-mG2a-f for analyzing the golden hamster-derived tissues and cells for SARS-CoV-2 research.