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Insight into Isolation and Elucidation of Cytotoxic Ergostanoids from the Mushroom Sarcosphaera Crassa (Santi) Pouzar: An Edible Mushroom

2022; RELX Group (Netherlands); Linguagem: Inglês

10.2139/ssrn.4003403

1556-5068

Zain Ullah, Mehmet Öztürk, Abdülselam Ertaş, Atia‐tul Wahab, Riadh Ben Mansour, M. Iqbal Choudhary,

Phytochemicals and Antioxidant Activities

Sarcosphaera crassa is a mushroom and consumed in Europe and Anatolia after cooked well. Thy cytotoxic activity of the extracts of unbaked S . crassa against MCF7, HT29, and HeLa cancer cell lines and toxicity against PDF fibroblast healthy cell lines were studied using MTT assay. Acetone and methanol extracts of mushroom exhibited significant cytotoxic activity. Further investigation of cytotoxic extracts afforded two new fatty acid sterols ( 1 - 2 ), a new ergosterol glycoside ( 4 ), and seven known compounds including a fatty acid sterol ( 3 ), a steroid glycoside ( 5 ), two ergostanoids ( 6 - 7 ) and three sugars ( 8 - 10 ). These compounds were identified as brassicasteryl heptadecanoate ( 1 ), brassicasteryl palmitoleate ( 2 ), brassicasteryl linoleate ( 3 ), brassicasterol β - ᴅ -xylofuranoside ( 4 ), brassicasterol β - ᴅ -glucoside ( 5 ), brassicasterol ( 6 ), ergosterol-endoperoxide ( 7 ), mannitol ( 8 ), erythritol ( 9 ) and turanose ( 10 ). Among them, 7 exhibited a moderate cytotoxic activity against HeLa (IC 50 :70.1±2.0 µg/mL) and high activity against HT29 (IC 50 :38.8±0.9 µg/mL), and MCF7 (IC 50 :62.9±1.3 µg/mL) cancer cell lines. Compounds 4 , 5, and 6 also exhibited significant cytotoxic activity against HT29 and MCF7. Moreover, all compounds exhibited weak toxicity against PDF healthy cell lines. This study indicates potential use of Sarcosphaera crassa as a natural source of cytotoxic ergostanoids, which can be considered as dietary supplement for cancer prevention.