First Report of Anthracnose Disease on Bletilla striata Caused by Colletotrichum orchidophilum in Yunnan, China
2021; American Phytopathological Society; Volume: 106; Issue: 3 Linguagem: Inglês
10.1094/pdis-07-21-1510-pdn
ISSN1943-7692
AutoresKuan Yang, H. L. Wang, Kaiyue Ye, Changqing Luo, Zaichao Wei, Hongping Huang, Shusheng Zhu, Liwei Guo, Xiaowei He,
Tópico(s)Plant and animal studies
ResumoHomePlant DiseaseVol. 106, No. 3First Report of Anthracnose Disease on Bletilla striata Caused by Colletotrichum orchidophilum in Yunnan, China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Anthracnose Disease on Bletilla striata Caused by Colletotrichum orchidophilum in Yunnan, ChinaK. Yang, H. L. Wang, K. H. Ye, C. Luo, Z. X. Wei, H. P. Huang, S. S. Zhu, L. W. Guo, and X. H. HeK. Yanghttps://orcid.org/0000-0003-0257-6081State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, H. L. Wanghttps://orcid.org/0000-0001-7768-8753State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, K. H. YeState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, C. LuoState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, Z. X. WeiState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, H. P. HuangState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, S. S. ZhuState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, L. W. Guo†Corresponding authors: X. H. He; E-mail Address: [email protected], and L. W. Guo; E-mail Address: [email protected]https://orcid.org/0000-0002-1319-6415State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China, and X. H. He†Corresponding authors: X. H. He; E-mail Address: [email protected], and L. W. Guo; E-mail Address: [email protected]State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaKey Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaSouthwest Forestry University, Kunming, Yunnan 650224, ChinaAffiliationsAuthors and Affiliations K. Yang1 2 H. L. Wang1 2 K. H. Ye1 2 C. Luo1 2 Z. X. Wei1 2 H. P. Huang1 2 S. S. Zhu1 2 L. W. Guo1 2 † X. H. He1 2 3 † 1State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China 2Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China 3Southwest Forestry University, Kunming, Yunnan 650224, China Published Online:9 Feb 2022https://doi.org/10.1094/PDIS-07-21-1510-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBletilla striata (Thunb.) Reichb. f. has high ornamental and medicinal value. B. striata has been used in Chinese medicine for thousands of years to treat various diseases, such as peptic ulcers, gastrointestinal diseases, lung diseases, and traumatic bleeding (Jiang et al. 2021). In September 2020, about 30% of B. striata plants in a plantation in Lancang County (Puer City, Yunnan Province, 22°48′17″ N, 99°46′58″ E) were found with symptoms resembling Bletilla anthracnose disease. In May 2021, symptomatic leaves were observed again with a disease incidence rate of 54.05% and collected. The initial symptoms were the appearance of one or more round light brown spots on the leaves. Subsequently, the lesions became larger, forming oval brown-black spots. On severely affected leaves, several spots coalesced, causing withering and death of the leaves. To isolate the pathogen, 10 symptomatic tissues (5 mm2) of 10 infected leaves were disinfected in 75% ethanol for 30 s followed by 2% sodium hypochlorite for 3 min, rinsed with sterile water three times, and incubated on potato dextrose agar (PDA) plates at 25°C for 5 to 7 days with a 12 h light/dark cycle. Finally, 10 pathogen strains with similar morphology were isolated and pure cultures were obtained by single spore isolation. The morphology of fungal colonies was round, carpet-like, flat and neat, and gray black. The conidiophores were colorless to brown and oval or stick-shaped. The conidia were cylindrical or oblong, consisted of a single cell with blunt round ends, and were on average 12.92 (8.13 to 21.21) μm × 3.96 (2.55 to 5.90) μm (n = 200). Based on the morphology and conidial characteristics, the pathogen was identified as Colletotrichum sp. (Damm et al. 2012). The total genomic DNA of representative isolates (LCTJ-02, LCTJ-03, LCTJ-04, LCTJ-05, and LCTJ-06) was extracted and the ITS region, GAPDH, ACT, and HIS3 gene regions were amplified and sequenced using the primer pairs ITS1/ITS4, GDF1/GDR1, 512F/783R, and CylH3F/CylH3R, respectively (Cai et al. 2009). The sequences were deposited in GenBank (MZ433189 to MZ433193 for ITS, MZ436430 to MZ436434 for GAPDH, MZ436435 to MZ436439 for HIS3, and MZ448474 to MZ448478 for ACT). BLAST search revealed that all sequences showed 98 to 100% homology with the corresponding sequences of Colletotrichum orchidophilum ex-type (CBS 632.80). Phylogenetic trees were constructed using neighbor-joining and maximum likelihood methods for the combined data set of the ITS, GAPDH, ACT, and HIS3 genes by MEGA-X (Kumar et al. 2018). In general, LCTJ-02, LCTJ-03, LCTJ-04, LCTJ-05, LCTJ-06, and C. orchidophilum type strains clustered on the same clade. To confirm pathogenicity, the five isolates of C. orchidophilum were inoculated in healthy leaves of potted B. striata, as described by Cai et al. (2009), with slight modifications. Each leaf was inoculated with three drops (10 µl.drop−1) of conidia suspension (106 spores.ml−1). The control group was mock inoculated with sterile water the same way. All samples were covered with plastic bags and maintained at 70 to 80% relative humidity for 7 days. After this period, all inoculated leaves showed similar lesions, and the symptoms were identical to those observed in the field. The control plants remained healthy. The pathogens were reisolated from two leaves of each treatment and reidentified as C. orchidophilum. To our knowledge, this is the first report of C. orchidophilum causing anthracnose on B. striata in China. In the future, the occurrence and transmission of this pathogen should be further studied in order to develop reasonable control measures.The author(s) declare no conflict of interest.References:Cai, L., et al. 2009. Fungal Divers. 39:183. https://www.fungaldiversity.org/fdp/sfdp/FD39-8.pdf ISI, Google ScholarDamm, U., et al. 2012. Stud. Mycol. 73:37. https://doi.org/10.3114/sim0010 Crossref, ISI, Google ScholarJiang, S., et al. 2021. J. Ethnopharmacol. 280:114263. https://doi.org/10.1016/j.jep.2021.114263 Crossref, Google ScholarKumar, S., et al. 2018. Mol. Biol. Evol. 35:1547. https://doi.org/10.1093/molbev/msy096 Crossref, ISI, Google ScholarK. Yang and H. L. Wang contributed equally.Funding: This work was supported by China Agriculture Research System (CARS-21), Major Science and Technology Project of Yunnan and Kunming (202102AE090042 and 20212021JH002).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 3 March 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 30 Mar 2022Published: 9 Feb 2022First Look: 13 Sep 2021Accepted: 8 Sep 2021 Page: 1070 Information© 2022 The American Phytopathological SocietyFundingChina Agriculture Research SystemGrant/Award Number: CARS-21Major Science and Technology Project of Yunnan and KunmingGrant/Award Number: 202102AE090042Grant/Award Number: 20212021JH002KeywordsBletilla striataanthracnoseColletotrichum orchidophilumThe author(s) declare no conflict of interest.PDF downloadCited byFirst report of Colletotrichum spaethianum causing anthracnose disease on Bletilla striata21 February 2023 | Canadian Journal of Plant Pathology
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