First Report of Fusarium temperatum Causing Fruit Blotch of Capsicum pubescens in Puebla, México
2021; American Phytopathological Society; Volume: 106; Issue: 6 Linguagem: Inglês
10.1094/pdis-09-21-1941-pdn
ISSN1943-7692
AutoresMarco Antonio Kevin Pérez‐Vázquez, L. A. Morales‐Mora, Omar Romero‐Arenas, Antonio Rivera, Gerardo Landeta‐Cortés, Nemesio Villa‐Ruano,
Tópico(s)Mycotoxins in Agriculture and Food
ResumoHomePlant DiseaseVol. 106, No. 6First Report of Fusarium temperatum Causing Fruit Blotch of Capsicum pubescens in Puebla, México PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Fusarium temperatum Causing Fruit Blotch of Capsicum pubescens in Puebla, MéxicoM. A. K. Pérez-Vázquez, L. A. Morales-Mora, O. Romero-Arenas, A. Rivera, G. Landeta-Cortés, and N. Villa-RuanoM. A. K. Pérez-VázquezCentro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla (BUAP), Km 1.7, Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, CP 72960, Puebla, México, L. A. Morales-MoraCentro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla (BUAP), Km 1.7, Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, CP 72960, Puebla, México, O. Romero-Arenashttps://orcid.org/0000-0003-0076-3609Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla (BUAP), Km 1.7, Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, CP 72960, Puebla, México, A. RiveraCentro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla (BUAP), Km 1.7, Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, CP 72960, Puebla, México, G. Landeta-CortésCentro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Prolongación de la 24 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72570 Puebla, México, and N. Villa-Ruano†Corresponding author: N. Villa-Ruano; E-mail Address: [email protected]https://orcid.org/0000-0002-0787-374XCONACyT-Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Prolongación de la 24 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72570 Puebla, MéxicoAffiliationsAuthors and Affiliations M. A. K. Pérez-Vázquez1 L. A. Morales-Mora1 O. Romero-Arenas1 A. Rivera1 G. Landeta-Cortés2 N. Villa-Ruano3 † 1Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla (BUAP), Km 1.7, Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, CP 72960, Puebla, México 2Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Prolongación de la 24 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72570 Puebla, México 3CONACyT-Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Prolongación de la 24 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72570 Puebla, México Published Online:21 Apr 2022https://doi.org/10.1094/PDIS-09-21-1941-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePuebla is one of the provinces in Mexico that produces manzano peppers (Capsicum pubescens). Recently, the producers of manzano peppers from the northern highlands of Puebla reported an aggressive fruit blotch that caused severe damage and substantial economic losses. Symptoms included rot spots with mycelial growth at the center of the spot, necrosis in the periphery, a change in fruit size, loss of turgor, and discoloration. The symptoms were observed at different fruit ripening stages. Around 50% of fruits (n = 50) were infected. Diseased fruits were collected from March to September 2020 in Yaonáhuac Puebla, Mexico. These samples were sanitized (20% sodium hypochlorite for 20 min) and stored in humidity chambers (n = 20) for 10 days at 28°C and 70% relative humidity to identify the causal agent of rot. Two fungal isolates were isolated and purified in potato dextrose agar (PDA), and the isolate with the less abundant mycelium was identified as Cylindrobasidium torrendii by ITS markers. C. torrendii did not exert pathogenic activity, whereas the second isolate had evident pathogenic properties. Morphological identification of the causal agent was completed from the monosporic cultures of the second isolate developed in PDA (Tariq et al. 2018). The fungus showed white and cottony mycelium, which became pinkish-white after 10 days of incubation, and it turned the culture medium to a dark-violet color. Koch's postulates were conducted with 1,000 conidia inoculated in the pericarp of sanitized asymptomatic peppers by mechanical penetration using a Hamilton syringe (n = 20; 28°C and 70% relative humidity for 12 days). After 6 days, the samples inoculated with the conidia showed the same symptoms as those observed under the field conditions. Microscopic characteristics were obtained from PDA and Spezieller Nährstoffarmer agar. The fungus showed hyaline mycelium with septate hyphae containing erect-branched conidiophores with one to three phialides. The size of macroconidia and microconidia (n = 100) was 20.3 to 43.1 μm and 6.2 to 14.3 µm, respectively (Pintos et al. 2013). Molecular analysis was done according to the method described by Morales-Mora et al. (2019) by sequencing the translation elongation factor 1 alpha (EF-1α) (EF-1H, ATGGGTAAGGAAGACAAGAC; EF-2T, GGAAGTACCAGTGATCATGTT) (Molnár et al. 2015; Scauflaire et al. 2011). The amplicon (671 bp) was sequenced with the oligonucleotide EF-2T and compared with the sequences of the accession numbers HM067686, HM067687 (Scauflaire et al. 2011), and KT447148 (Lanza 2016) of the National Center for Biotechnological Information (NCBI), which showed 100% homology with Fusarium temperatum. The partial sequence of EF-1α from F. temperatum isolated in this study was deposited at NCBI databank with the accession number MW570680. Because F. temperatum has been reported as the infective agent of maize around the world (Pintos et al. 2013), F. temperatum causing fruit blotch can be a new threat to the producers of manzano peppers in Mexico. The identification of this pathogen is the first important step to develop management strategies for the control of this disease.The author(s) declare no conflict of interest.References:Lanza, F. E. 2016. Plant Dis. 100:1019. https://doi.org/10.1094/PDIS-11-15-1301-PDN Link, ISI, Google ScholarMolnár, O., et al. 2015. Acta Microbiol. Immunol. Hung. 62:109. https://doi.org/10.1556/030.62.2015.2.2 Crossref, ISI, Google ScholarMorales-Mora, L. A., et al. 2019. Plant Dis. 103:2668. https://doi.org/10.1094/PDIS-05-19-1010-PDN Link, ISI, Google ScholarPintos, C., et al. 2013. Plant Dis. 97:1252. Google ScholarScauflaire, J., et al. 2011. Mycologia 103:586. https://doi.org/10.3852/10-135 Crossref, ISI, Google ScholarTariq, A., et al. 2018. Plant Dis. 102:2645. https://doi.org/10.1094/PDIS-02-18-0221-PDN Link, ISI, Google ScholarFunding: Funding was provided by CONACyT-México, PRODEP-SEP-México and the Benemerita Universidad Autónoma de Puebla.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 6 June 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 2 Jun 2022 Published: 21 Apr 2022 First Look: 16 Nov 2021 Accepted: 12 Nov 2021 Page: 1758 Information© 2022 The American Phytopathological SocietyFundingCONACyT-MéxicoPRODEP-SEP-MéxicoBenemerita Universidad Autónoma de PueblaKeywordsCapsicum pubescensFusarium temperatumPuebla-MéxicoThe author(s) declare no conflict of interest.PDF downloadCited byFirst Report of Vascular Wilt on Peanut in Mexico Caused by Fusarium incarnatumOmar Romero-Arenas, Petra Andrade-Hoyos, Hilda V. Silva-Rojas, Alfonso Luna-Cruz, and Saira J. 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