First Report of Puccinia thaliae Causing Leaf Rust on Canna indica in Malaysia
2021; American Phytopathological Society; Volume: 106; Issue: 6 Linguagem: Inglês
10.1094/pdis-10-21-2332-pdn
ISSN1943-7692
AutoresYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shifang Li, Khim Phin Chong,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 106, No. 6First Report of Puccinia thaliae Causing Leaf Rust on Canna indica in Malaysia PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Puccinia thaliae Causing Leaf Rust on Canna indica in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin ChongYing Wei Khoohttps://orcid.org/0000-0003-0927-4289State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100191, ChinaFaculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, MalaysiaSearch for more papers by this author, Hui Teng Tanhttps://orcid.org/0000-0002-6814-599XKey Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaSearch for more papers by this author, Yam Sim Khawhttps://orcid.org/0000-0002-5867-998XKey Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaSearch for more papers by this author, Shi-Fang Lihttps://orcid.org/0000-0003-4614-1768State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100191, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaSearch for more papers by this author, and Khim Phin Chong†Corresponding author: K. P. Chong; E-mail Address: chongkp@ums.edu.myhttps://orcid.org/0000-0002-5143-7283Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, MalaysiaSearch for more papers by this authorAffiliationsAuthors and Affiliations Ying Wei Khoo1 2 Hui Teng Tan3 Yam Sim Khaw3 Shi-Fang Li1 4 Khim Phin Chong2 † 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100191, China 2Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, Malaysia 3Key Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia 4Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China Published Online:11 May 2022https://doi.org/10.1094/PDIS-10-21-2332-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleCanna indica L. (family Cannaceae), locally known as bunga kana, is a perennial plant grown as a source of starch and for ornamental purposes in Malaysia. During June 2021, bunga kana with rust symptoms and signs were collected from the Universiti Malaysia Sabah in the province of Sabah. The severity was 95%, and the incidence was 90%. Yellow uredinia were observed primarily on the abaxial surface of the leaves. As the disease progressed, leaves were covered with coalescing pustules, and chlorosis and brown necrosis developed. Microscopic examination of pustules revealed the presence of urediniospores and teliospores. Urediniospores were round to ovoid in shape, yellow, echinulate, 17.7 to 24.6 × 26.8 to 45.2 μm, with two equatorial pores. Teliospores were elongate-clavate, with a rounded apex, yellow contents, 18.3 × 20.2 to 45.8 × 53.9 μm, with a short pedicel. Yellow urediniospores were collected using a fine brush, and genomic DNA was extracted using a lysis buffer (Tris-HCl [0.1 M, pH 9.5], NaCl [1 M], and EDTA [0.5 M, pH 8]) prior to heating at 95°C for 10 min. KOD One PCR Master Mix containing hot-start modified KOD DNA polymerase was used for PCR amplification. The 28S ribosomal RNA gene was amplified using primers Rust28SF (Aime et al. 2018) and LR5 (Vilgalys and Hester 1990). PCR products with positive amplicons were sent to Apical Scientific Sdn. Bhd. for sequencing. BLASTn analysis of the newly generated 28S ribosomal RNA gene (OK462969) in GenBank revealed a 99% sequence identity to Puccinia thaliae Dietel (JX206994 of 28S ribosomal RNA gene). The morphological and molecular characterization of the rust fungus matched P. thaliae described by Padamsee and McKenzie (2012). Koch’s postulates were performed with spray inoculations of urediniospores suspended in water (106 spores/ml) on leaves of three healthy bunga kana plants, while water was sprayed on three additional bunga kana plants as a control. The inoculated bunga kana plants were covered with plastic for 48 h at 25°C in the dark and then placed in a greenhouse. Symptoms and signs similar to those of the field collection occurred after 13 days postinoculation. No symptoms occurred on controls. Leaf rust on bunga kana plants caused by P. thaliae has been reported in Europe (Talhinhas et al. 2016), Hawaii (Nelson 2013), India (Gopi et al. 2014), Mexico (Cedas de Jesús et al. 2018), Nepal (Adhikari and Durrieu 2016), New Zealand (Padamsee and McKenzie 2012), Singapore (Neo and Tham 2010), and South Africa (van Jaarsveld et al. 2006) over the past 15 years. To our knowledge, this is the first report of P. thaliae causing leaf rust on C. indica in Malaysia. Our findings expand the geographic range of P. thaliae and indicate it could be a potential threat that limits the starch production of C. indica in Malaysia.The author(s) declare no conflict of interest.References:Adhikari, M. K., and Durrieu, G. 2016. Bull. Dept. Pl. Res. 38:42. Google ScholarAime, M. C., et al. 2018. Stud. Mycol. 89:143. https://doi.org/10.1016/j.simyco.2018.02.002 Crossref, ISI, Google ScholarCedas de Jesús, M., et al. 2018. S.F. J. Mycol. 1:1. Google ScholarGopi, R., et al. 2014. J. Mycopathol. Res. 52:155. Google ScholarNelson, S. 2013. Rust of Canna Lily. College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa. https://www.ctahr.hawaii.edu/oc/freepubs/pdf/PD-96.pdf Google ScholarNeo, N. W., and Tham, F. Y. 2010. Plant Pathol. 59:406. https://doi.org/10.1111/j.1365-3059.2009.02194.x Crossref, Google ScholarPadamsee, M., and McKenzie, E. H. 2012. Australas. Plant Dis. 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SDK0079-2019).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 6 June 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 2 Jun 2022Published: 11 May 2022First Look: 29 Nov 2021Accepted: 24 Nov 2021 Page: 1760 Information© 2022 The American Phytopathological SocietyFundingUniversiti Malaysia SabahGrant/Award Number: SDK0079-2019KeywordsCanna indicafield cropsfungiMalaysiaornamentalspathogen detectionPuccinia thaliaeyield loss and economic impactsThe author(s) declare no conflict of interest.PDF downloadCited byFirst Report of Colletotrichum siamense Causing Anthracnose on Crinum asiaticum in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong7 October 2022 | Plant Disease, Vol. 106, No. 11First Report of Neopestalotiopsis cubana Causing Leaf Blight on Ixora chinensis in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong31 August 2022 | Plant Disease, Vol. 106, No. 10First Report of Anthracnose on ‘Purple Dream’ Solanum melongena in Malaysia Caused by Colletotrichum siamenseYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong8 August 2022 | Plant Disease, Vol. 106, No. 9First Report of Lasiodiplodia theobromae Causing Leaf Spot on Aloe vera var. chinensis in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong20 July 2022 | Plant Disease, Vol. 106, No. 8First report of Epicoccum sorghinum causing leaf spot on Basella alba in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong22 July 2022 | Plant Disease, Vol. 0, No. jaFirst Report of Leaf Spot on Basella rubra Caused by Fusarium proliferatum in MalaysiaYing Wei Khoo, Yam Sim Khaw, Hui Teng Tan, Shi-Fang Li, and Khim Phin Chong22 July 2022 | Plant Disease, Vol. 0, No. jaFirst Report of Fusarium oxysporum Causing leaf spot on Basella rubra in MalaysiaYing Wei Khoo, Yam Sim Khaw, Hui Teng Tan, Shi-Fang Li, and Khim Phin Chong19 June 2022 | Plant Disease, Vol. 0, No. jaFirst Report of Neoscytalidium dimidiatum Causing stem canker on Selenicereus megalanthus in MalaysiaYing Wei Khoo, Hui Teng Tan, Yam Sim Khaw, Shi-Fang Li, and Khim Phin Chong31 May 2022 | Plant Disease, Vol. 0, No. jaFirst Report of Stem brown spot on ‘Thai Gold’ Selenicereus megalanthus in Malaysia caused by Nigrospora sphaerica Ying Wei Khoo, Yam Sim Khaw, Hui Teng Tan, Shi-Fang Li, and Khim Phin Chong17 May 2022 | Plant Disease, Vol. 0, No. jaFirst report of Macrophomina phaseolina causing leaf blight on Pometia pinnata in MalaysiaYing Wei Khoo, Yam Sim Khaw, Hui Teng Tan, Shi-Fang Li, and Khim Phin Chong17 May 2022 | Plant Disease, Vol. 0, No. ja
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