First Report of Colletotrichum fructicola Causing Anthracnose on Phoebe sheareri in China
2021; American Phytopathological Society; Volume: 106; Issue: 7 Linguagem: Inglês
10.1094/pdis-08-21-1841-pdn
ISSN1943-7692
AutoresXiaoqiao Huang, Yan Wu, Yuan Li, Haiping Lin, Ma LiangJin, Xiu Su, Xudong Zhou,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 106, No. 7First Report of Colletotrichum fructicola Causing Anthracnose on Phoebe sheareri in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Colletotrichum fructicola Causing Anthracnose on Phoebe sheareri in ChinaXiaoqiao Huang, Yan Wu, Yuan Li, Haiping Lin, Liangjin Ma, Xiu Su, and Xudong ZhouXiaoqiao HuangState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China, Yan WuState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, ChinaDepartment of Plant Pathology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China, Yuan LiState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China, Haiping LinState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China, Liangjin MaState Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China, Xiu Su†Corresponding authors: X. Su; E-mail Address: suxiu@zafu.edu.cn, and X. D. Zhou; E-mail Address: xudongzhou@zafu.edu.cnhttps://orcid.org/0000-0001-7492-1580State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China, and Xudong Zhou†Corresponding authors: X. Su; E-mail Address: suxiu@zafu.edu.cn, and X. D. Zhou; E-mail Address: xudongzhou@zafu.edu.cnhttps://orcid.org/0000-0002-9580-9093State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, ChinaAffiliationsAuthors and Affiliations Xiaoqiao Huang1 Yan Wu1 2 Yuan Li1 Haiping Lin1 Liangjin Ma1 Xiu Su1 † Xudong Zhou1 † 1State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China 2Department of Plant Pathology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China Published Online:23 May 2022https://doi.org/10.1094/PDIS-08-21-1841-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePhoebe sheareri (Hemsl.) Gamble is a high-value timber tree species with a wide range of uses, including for landscape afforestation because of its beautiful shape and luxuriant branches. Previous studies found that Neofusicum parvum can cause ulceration and necrosis on the main stems of P. sheareri (Chen et al. 2019) and branch wilt of P. zhennan (Zhu et al. 2019). In September 2019, anthracnose was observed on P. sheareri leaves in Lishui, Zhejiang province, China. The diseased leaves are characterized by dark brown lesions. The infection usually starts from the leaf tip or edge, and then the infected leaves turn yellow, wither, and finally fall off. The infection sometimes occurs on small twigs, causing the whole branch to wither. Plants from 15 plantations were surveyed, and the disease incidence was about 30%. Fifteen freshly infected leaves were collected and cut into small pieces (5 × 5 mm), sterilized in 75% ethanol solution for 30 s, and washed with sterile water three times. They were further sanitized in 2% sodium hypochlorite solution for 2 to 3 min and dried in sterile filter paper after being washed with sterile water another three times. Leaf pieces were transferred to potato dextrose agar (PDA) plates and incubated at 25°C in the dark for 3 days. The fungal cultures were purified and a typical isolate (TJ01) was selected for further morphological characterization and DNA sequence comparison. The strain was initially grayish white, villous, and posteriorly grayish green, and it produced orange red spore clumps. Spores were single celled after maturation, oblong, and colorless, and 8.5 to 19.5 × 4.5 to 5.5 μm. Morphological characteristics were consistent with those in the genus Colletotrichum. DNA of the isolate was extracted, and sequence data were obtained and compared with those downloaded from GenBank. The internal transcribed spacer (ITS), intronic glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), beta-tubulin (TUB2), and calmodulin (CAL) genes were partially amplified and sequenced using the primer pairs ITS-1 + ITS-4, GDF + GDR, ACT-512F + ACT-783R, T1 + Bt2b, and CL1C + Cl2C, respectively (Fu et al. 2019). The resulting nucleotide sequences were individually subjected to BLAST searches in GenBank. The nucleotide sequences of ITS (MZ088144), GAPDH (MZ133607), ACT (MZ133608), TUB2 (MZ133609), and CAL (MZ133610) of the isolate showed 99.83% similarity to the ITS sequence (MN829453), 98.24% similarity to the GAPDH sequence (MN525875), 98.94% similarity to the ACT sequence (MK341539), 100% similarity to the TUB2 sequence (MG657352), and 100% similarity to the CAL sequence (MN525839). A phylogenetic tree was constructed using MEGA 7 based on the tandem of five genes (ITS-GAPDH-ACT-TUB2-CAL). It revealed that the isolate resides in the clade of C. fructicola species. Inoculation was done on leaves of 10 P. sheareri plants in the field to verify its pathogenicity. Three healthy leaves of each plant were surface sterilized with 75% ethanol and dried. The leaves were punctured using a sterilized needle, inoculated with 5-mm-diameter PDA plugs excised from 7-day-old cultures, and wrapped with Parafilm. Nine pieces of healthy leaves inoculated with sterilized PDA plugs served as controls. Disease symptoms developed on all the C. fructicola-inoculated leaves 5 days after inoculation, and a yellow brown lesion became apparent 16 days later, whereas the control leaves remained asymptomatic. C. fructicola was reisolated from the lesions, but not from the control leaves, fulfilling Koch's postulates. This fungus is a well-known pathogen and has led to anthracnose on many plant species globally. However, our study is the first report of C. fructicola causing anthracnose on P. sheareri worldwide, and its potential threat should be evaluated.The author(s) declare no conflict of interest.References:Chen, Y., et al. 2019. Plant Dis. 103:1780. https://doi.org/10.1094/PDIS-01-19-0006-PDN Link, Google ScholarFu, M., et al. 2019. Persoonia 42:1. https://doi.org/10.3767/persoonia.2019.42.01 Crossref, ISI, Google ScholarZhu, H. M. Y., et al. 2019. Plant Dis. 103:2135. https://doi.org/10.1094/PDIS-08-18-1319-PDN Link, Google ScholarFunding: This work was supported by General Scientific Research Projects of Zhejiang Province Department of Education (No. Y201431042).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 7 July 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 8 Jul 2022Published: 23 May 2022First Look: 2 Dec 2021Accepted: 30 Nov 2021 Page: 1994 Information© 2022 The American Phytopathological SocietyFundingZhejiang Province Department of EducationGrant/Award Number: Y201431042KeywordsColletotrichum fructicolaKoch's postulatesmorphological characteristicsPhoebe sheareriphylogenetic treeThe author(s) declare no conflict of interest.PDF downloadCited byPest categorisation of Colletotrichum fructicolaEFSA Journal, Vol. 19, No. 8
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