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Decellularization of porcine kidney with submicellar concentrations of SDS results in the retention of ECM proteins required for the adhesion and maintenance of human adult renal epithelial cells

2022; Royal Society of Chemistry; Volume: 10; Issue: 11 Linguagem: Inglês

10.1039/d1bm01017d

2047-4849

Tonya Bongolan, Jennifer Whiteley, Jorge Castillo-Prado, Amanda Fantin, Brett Larsen, Cassandra J. Wong, Laura Mazilescu, Masataka Kawamura, Peter Urbanellis, Anna Jonebring, Eric W. Salter, Graham Collingridge, Rebecca Gladdy, Ryan Hicks, Anne‐Claude Gingras, Markus Selzner, Ian M. Rogers,

Electrospun Nanofibers in Biomedical Applications

When decellularizing kidneys, it is important to maintain the integrity of the acellular extracellular matrix (ECM), including associated adhesion proteins and growth factors that allow recellularized cells to adhere and migrate according to ECM specificity. Kidney decellularization requires the ionic detergent sodium dodecyl sulfate (SDS); however, this results in a loss of ECM proteins important for cell adherence, migration, and growth, particularly glycosaminoglycan (GAG)-associated proteins. Here, we demonstrate that using submicellar concentrations of SDS results in a greater retention of structural proteins, GAGs, growth factors, and cytokines. When porcine kidney ECM scaffolds were recellularized using human adult primary renal epithelial cells (RECs), the ECM promoted cell survival and the uniform distribution of cells throughout the ECM. Cells maintained the expression of mature renal epithelial markers but did not organize on the ECM, indicating that mature cells are unable to migrate to specific locations on ECM scaffolds.