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Multicomponent Hydrogel Matrices of Fmoc‐FF and Cationic Peptides for Application in Tissue Engineering

2022; Wiley; Volume: 22; Issue: 7 Linguagem: Inglês

10.1002/mabi.202200128

1616-5195

Elisabetta Rosa, Carlo Diaferia, Eliana Gianolio, Teresa Sibillano, Enrico Gallo, Giovanni Smaldone, Mariano Stornaiuolo, Cinzia Giannini, Giancarlo Morelli, Antonella Accardo,

RNA Interference and Gene Delivery

In the last years, peptide-based hydrogels are being increasingly used as suitable matrices for biomedical and pharmaceutical applications, including drug delivery and tissue engineering. Recently, the synthesis and the gelation properties of a small library of cationic peptides, containing a Lys residue at the C-terminus and derivatized with an Fmoc group or with the fluorenyl methoxycarbonyl-diphenylalanine (FmocFF) at the N-terminus are derived. Here, it is demonstrated that the combination of these peptides with the well-known hydrogelator FmocFF, in different weight/weight ratios, allows the achievement of seven novel self-sorted hydrogels, which share similar peptide organization of their supramolecular matrix. Rheological and relaxometric characterization highlight a different mechanical rigidity and water mobility in the gels as demonstrated by the storage modulus values (200 Pa < G' < 35 000 Pa) and by relaxometry, respectively. In vitro studies demonstrate that most of the tested mixed hydrogels do not disturb significantly the cell viability (>95%) over 72 h of treatment. Moreover, in virtue to its capability to strongly favor adhesion, spreading and duplication of 3T3-L1 cells, one of the tested hydrogel may be eligible as synthetic extracellular matrix.