Correction
2001; Lippincott Williams & Wilkins; Volume: 21; Issue: 10 Linguagem: Inglês
10.1161/atvb.21.10.1706
ISSN1524-4636
ResumoHomeArteriosclerosis, Thrombosis, and Vascular BiologyVol. 21, No. 10Correction Free AccessCorrectionPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toFree AccessCorrectionPDF/EPUBCorrection Originally published15 Mar 2018https://doi.org/10.1161/atvb.21.10.1706Arteriosclerosis, Thrombosis, and Vascular Biology. 2001;21:1706This article corrects the followingInhibition of Arteriosclerosis by T-Cell Depletion in Normocholesterolemic Rabbits Immunized With Heat Shock Protein 65We published “Inhibition of Arteriosclerosis by T-Cell Depletion in Normocholesterolemic Rabbits Immunized With Heat Shock Protein 65” (Arterioscler Thromb Vasc Biol. 1999;19:1905-1911) under the consideration that treatment of normocholesterolemic rabbits with a monoclonal antibody against rabbit T-cells prevents the development of arteriosclerosis after immunization with mycobacterial heat shock protein 65. The antibody used for this study was produced from the hybridoma line L11/135 obtained from the American Type Culture Collection (ATCC-No. TIB 188). In the ATCC specification, this hybridoma was described as producing antibodies reactive with rabbit T-cells. In the reference on this clone given in the ATCC specification sheet,1 a panel of six monoclonal antibodies used against cell surface glycoproteins of a rabbit T-lymphocyte line was studied. This paper explicitly states that in functional studies only the L11/135-bearing cells responded to the T-cell mitogens concanavalin A and phytohemagglutinin and to allogenic splenocytes.However, we became aware recently that this antibody, defined as pan-T-specific, recognized the equivalent of CD43/leukosialin,2 an antigen that is strongly expressed by all T-cells, but also weakly stains monocytes and macrophages. Although the experimental data that we presented (ie, the severe quantitative and functional suppression of T-cells in the antibody-treated animals) are solid and reproducible, we are at present not able to assign the atherosclerosis-inhibiting effect to either a depletion of T-cells or the inhibition of the adhesion to and subsequent transmigration of monocytes through the vascular endothelium.3 CD3 and CD43 are jointly present in a large complex in a mild detergent lysate of T-cells, and it is suggested to add this as a co-stimulatory molecule in CD3/T-cell receptor signalling.4 Unfortunately, a pure anti-rabbit-CD3 monoclonal antibody that binds to extra cellular domains of the CD3-complex and can therefore be used for in vivo experiments is not available. We apologize for any confusion this may have caused.Bernhard Metzler, Manuel Mayr,Hermann Dietrich, Mahauir Singh,Evelyn Wiebe, Qingbo Xu, George Wick References 1 Jackson S, Chused TM, Wilkinson JM, Leiserson WM, Kindt TJ. Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. J Exp Med. 1983; 157: 34–46.CrossrefMedlineGoogle Scholar2 Wilkinson JM, Galea-Lauri J, Sellars RA, Boniface C. Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies. Immunology. 1992; 76: 625–630.MedlineGoogle Scholar3 McEvoy LM, Jutila MA, Tsao PS, Cooke JP, Butcher EC. Anti-CD43 inhibits monocytes-endothelial adhesion in inflammation and atherogenesis. Blood. 1997; 9: 3587–3594.Google Scholar4 Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995; 25: 1051–1055.CrossrefMedlineGoogle Scholar Previous Back to top Next FiguresReferencesRelatedDetailsCited By Henderson B, Rossmann A, Mayerl C, Wick M and Wick G Atherosclerosis—An Age-dependent Autoimmune Disease Handbook on Immunosenescence, 10.1007/978-1-4020-9063-9_53, (1063-1087) Wick G, Knoflach M and Xu Q (2004) Autoimmune and Inflammatory Mechanisms in Atherosclerosis, Annual Review of Immunology, 10.1146/annurev.immunol.22.012703.104644, 22:1, (361-403), Online publication date: 1-Apr-2004. Related articlesInhibition of Arteriosclerosis by T-Cell Depletion in Normocholesterolemic Rabbits Immunized With Heat Shock Protein 65Bernhard Metzler, et al. Arteriosclerosis, Thrombosis, and Vascular Biology. 1999;19:1905-1911 October 2001Vol 21, Issue 10 Advertisement Article InformationMetrics https://doi.org/10.1161/atvb.21.10.1706 Originally publishedMarch 15, 2018 PDF download Advertisement
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