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Molecular and modeling analyses of the structure and function of nitrifying activated sludge

1999; Pergamon Press; Volume: 39; Issue: 1 Linguagem: Inglês

10.2166/wst.1999.0012

1996-9732

Bruce E. Rittmann, Chrysi Laspidou, Jodi Flax, David A. Stahl, V. Urbain, Hervé Harduin, J.J. van der Waarde, Bert Geurkink, Maurice Henssen, H. Brouwer, A. Klapwijk, Maarten Wetterauw,

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Research Article| January 01 1999 Molecular and modeling analyses of the structure and function of nitrifying activated sludge Bruce E. Rittmann; Bruce E. Rittmann *Environmental Engineering Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3109, USA Search for other works by this author on: This Site PubMed Google Scholar Chrysi S. Laspidou; Chrysi S. Laspidou *Environmental Engineering Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3109, USA Search for other works by this author on: This Site PubMed Google Scholar Jodi Flax; Jodi Flax *Environmental Engineering Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3109, USA Search for other works by this author on: This Site PubMed Google Scholar David A. Stahl; David A. Stahl *Environmental Engineering Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3109, USA Search for other works by this author on: This Site PubMed Google Scholar Vincent Urbain; Vincent Urbain **Centre International de Recherche Sur l'Eau et l'Environnement, 38 rue du Président Wilson, Le Pecq 78230, France Search for other works by this author on: This Site PubMed Google Scholar Hervé Harduin; Hervé Harduin **Centre International de Recherche Sur l'Eau et l'Environnement, 38 rue du Président Wilson, Le Pecq 78230, France Search for other works by this author on: This Site PubMed Google Scholar Jaap J. van der Waarde; Jaap J. van der Waarde ***Bioclear Environmental Biotechnology, P.O. Box 2262, 9704 CG Groningen, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Bert Geurkink; Bert Geurkink ***Bioclear Environmental Biotechnology, P.O. Box 2262, 9704 CG Groningen, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Maurice J. C. Henssen; Maurice J. C. Henssen ***Bioclear Environmental Biotechnology, P.O. Box 2262, 9704 CG Groningen, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Harry Brouwer; Harry Brouwer †Sub-department of Environmental Technology, Department of Agricultural Environmental and Systems Technology, Wageningen Agricultural University, P.O. Box 81296700 EV Wageningen, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar ... Show more Abraham Klapwijk; Abraham Klapwijk †Sub-department of Environmental Technology, Department of Agricultural Environmental and Systems Technology, Wageningen Agricultural University, P.O. Box 81296700 EV Wageningen, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Maarten Wetterauw Maarten Wetterauw ‡Vertis b.v., P.O. Box 739640 AB Veendam, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar Water Sci Technol (1999) 39 (1): 51–59. https://doi.org/10.2166/wst.1999.0012 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Twitter LinkedIn Tools Icon Tools Cite Icon Cite Permissions Search Site Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThis Journal Search Advanced Search Citation Bruce E. Rittmann, Chrysi S. Laspidou, Jodi Flax, David A. Stahl, Vincent Urbain, Hervé Harduin, Jaap J. van der Waarde, Bert Geurkink, Maurice J. C. Henssen, Harry Brouwer, Abraham Klapwijk, Maarten Wetterauw; Molecular and modeling analyses of the structure and function of nitrifying activated sludge. Water Sci Technol 1 January 1999; 39 (1): 51–59. doi: https://doi.org/10.2166/wst.1999.0012 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Activated sludge community structure was assessed directly by oligonucleotide probes for seven treatment plants in France and two treatment plants in The Netherlands. Routine operating data also were obtained for these plants. Mathematical modeling was employed to translate the operating data into parameters that reflect community function and structure. These model-translated measures were compared to the direct probing measures of community structure. Extraction of RNA and subsequent slot-blot hybridization with probes Nso1225 (for active ammonium oxidizers) and Eub338 (for all active bacteria) confirmed that the ratio of active nitrifiers to active heterotrophs was mainly explained by the ratio of TKN:BODL removed and that probe Nso1225 provided a quantitative measure of ammonium oxidizers easily distinguishable from background. Fluorescent in situ hybridization (FISH) followed similar trends. The ratio of active nitrifiers to total VSS depended on the TKN/BODL removal ratio and the sludge age. Finally, the ng rRNA/mg VSS hybridized by probe Eub338 was strongly and inversely correlated to the sludge age and suggests that specific rRNA contents of about 11,000 ng rRNA/mg VSSactive for heterotrophic bacteria and 14,000 ng rRNA/mg VSSactive for ammonium oxidizers can be used to estimate active VSS from probing results targeted to rRNA. Active biomass, biological treatment, heterotrophs, microbial ecology, modeling, molecular analyses, nitrifiers, oligonucleotide probes This content is only available as a PDF. © IWA Publishing 1999 You do not currently have access to this content.