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Morphogenesis of Pollen Callus Cultures of Hyoscyamus niger

1983; Wiley; Volume: 70; Issue: 4 Linguagem: Inglês

10.2307/2443162

1537-2197

V. Raghavan, R. Nagmani,

Plant Reproductive Biology

American Journal of BotanyVolume 70, Issue 4 p. 524-531 Article MORPHOGENESIS OF POLLEN CALLUS CULTURES OF HYOSCYAMUS NIGER V. Raghavan, V. Raghavan Department of Botany, The Ohio State University, Columbus, Ohio, 43210Search for more papers by this authorR. Nagmani, R. Nagmani Department of Botany, The Ohio State University, Columbus, Ohio, 43210Search for more papers by this author V. Raghavan, V. Raghavan Department of Botany, The Ohio State University, Columbus, Ohio, 43210Search for more papers by this authorR. Nagmani, R. Nagmani Department of Botany, The Ohio State University, Columbus, Ohio, 43210Search for more papers by this author First published: 01 April 1983 https://doi.org/10.1002/j.1537-2197.1983.tb07879.xCitations: 3AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Morphogenesis of calluses of single pollen grain origin shed from anthers of Hyoscyamus niger cultured in a liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) was followed upon their transfer to a solid medium with or without 2,4-D. In a solid medium lacking 2,4-D, small calluses consisting of one to five nodular groups of cells at the time of inoculation differentiated root and shoot systems and formed miniature seedlings. In the same medium large calluses with several nodules initially formed a crop of bipolar somatic embryoids with well-defined root and shoot axes which subsequently differentiated into seedlings. Irrespective of their size at the time of transfer, calluses grown in a solid medium containing 2,4-D continued to proliferate without showing signs of organogenesis or embryogenesis. Citing Literature Volume70, Issue4April 1983Pages 524-531 RelatedInformation