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Late proliferation of retinal Müller cell progenitors facilitates preferential targeting with retroviral vectors in vitro

1997; Wiley; Volume: 20; Issue: 3 Linguagem: Inglês

10.1002/(sici)1520-6408(1997)20

1520-6408

Paul J. Linser, Burkhard Schloßhauer, Deni S. Galileo, William R. Buzzi, Rana C. Lewis,

Cytomegalovirus and herpesvirus research

Developmental GeneticsVolume 20, Issue 3 p. 186-196 Late proliferation of retinal Müller cell progenitors facilitates preferential targeting with retroviral vectors in vitro Paul J. Linser, Corresponding Author Paul J. Linser [email protected] Whitney Laboratory, University of Florida, GainesvilleWhitney Laboratory, 9505 Ocean Shore Blvd., St. Augustine, FL 32086.Search for more papers by this authorBurkhard Schlosshauer, Burkhard Schlosshauer Naturwissenschaftliches und Medizinisches Institut an der Universitat Tübingen, Reutlingen, GermanySearch for more papers by this authorDeni S. Galileo, Deni S. Galileo Department of Cellular Biology and Anatomy, Medical College of Georgia, AugustaSearch for more papers by this authorWilliam R. Buzzi, William R. Buzzi Whitney Laboratory, University of Florida, GainesvilleSearch for more papers by this authorRana C. Lewis, Rana C. Lewis Whitney Laboratory, University of Florida, GainesvilleSearch for more papers by this author Paul J. Linser, Corresponding Author Paul J. Linser [email protected] Whitney Laboratory, University of Florida, GainesvilleWhitney Laboratory, 9505 Ocean Shore Blvd., St. Augustine, FL 32086.Search for more papers by this authorBurkhard Schlosshauer, Burkhard Schlosshauer Naturwissenschaftliches und Medizinisches Institut an der Universitat Tübingen, Reutlingen, GermanySearch for more papers by this authorDeni S. Galileo, Deni S. Galileo Department of Cellular Biology and Anatomy, Medical College of Georgia, AugustaSearch for more papers by this authorWilliam R. Buzzi, William R. Buzzi Whitney Laboratory, University of Florida, GainesvilleSearch for more papers by this authorRana C. Lewis, Rana C. Lewis Whitney Laboratory, University of Florida, GainesvilleSearch for more papers by this author First published: 06 December 1998 https://doi.org/10.1002/(SICI)1520-6408(1997)20:3 3.0.CO;2-3Citations: 15AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract During vertebrate neural retina development, the relationship between mitotic activity in progenitor cells and the acquisition of a mature cell phenotype remains an area of controversy. The Müller glial cell has long been recognized as one of the last cell types of the retina to mature, which occurs under the influence of cell–cell interactions. In this report we examine the acquisition of the Müller cell phenotype in relation to mitotic activity. Using immunohistochemical markers, we demonstrate that a gene product characteristic of mature Müller cells, the 2M6 antigen, is expressed in mitotically active cells, even after all the major retina architectural features have been laid down. Furthermore, we show that retroviral infection, a process that requires mitotically active cells, preferentially targets Müller cell progenitors when late embryonic retina is infected in vitro. The two lines of evidence are consistent with a model for Müller cell differentiation that includes a mitotically active progenitor that has already begun to express specific differentiation gene products. Dev. Genet. 20:186–196, 1997. © 1997 Wiley-Liss, Inc. 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