Production of Monoclonal Antibodies
2006; Wiley; Volume: 74; Issue: 1 Linguagem: Inglês
10.1002/0471142735.im0205s74
ISSN1934-368X
AutoresWayne M. Yokoyama, Michelle Christensen, Gary Dos Santos, D M Miller,
Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoThis unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).
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