Portal de Periódicos da CAPES

Photodamage Induced by Zinc(II)-phthalocyanine to Microtubules, Actin, α-Actinin and Keratin of HeLa Cells¶

2001; Wiley; Volume: 73; Issue: 3 Linguagem: Inglês

10.1562/0031-8655(2001)073 2.0.co;2

1751-1097

Ángeles Juarranz, Jesús Espada, Juan C. Stockert, Ángeles Villanueva, Silvia Polo, Verónica Domínguez, Magdalena Cañete,

Porphyrin and Phthalocyanine Chemistry

Photochemistry and PhotobiologyVolume 73, Issue 3 p. 283-289 Photodamage Induced by Zinc(II)-phthalocyanine to Microtubules, Actin, α-Actinin and Keratin of HeLa Cells¶ Angeles Juarranz, Corresponding Author Angeles Juarranz Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain *To whom correspondence should be addressed at: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Canto Blanco, E-28049 Madrid, Spain. Fax: 34-91-397-8344;[email protected]Search for more papers by this authorJesús Espada, Jesús Espada Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorJuan Carlos Stockert, Juan Carlos Stockert Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorAngeles Villanueva, Angeles Villanueva Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorSilvia Polo, Silvia Polo Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorVerónica Domínguez, Verónica Domínguez Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorMagdalena Cañete, Magdalena Cañete Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this author Angeles Juarranz, Corresponding Author Angeles Juarranz Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain *To whom correspondence should be addressed at: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Canto Blanco, E-28049 Madrid, Spain. Fax: 34-91-397-8344;[email protected]Search for more papers by this authorJesús Espada, Jesús Espada Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorJuan Carlos Stockert, Juan Carlos Stockert Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorAngeles Villanueva, Angeles Villanueva Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorSilvia Polo, Silvia Polo Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorVerónica Domínguez, Verónica Domínguez Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this authorMagdalena Cañete, Magdalena Cañete Departamento de Biología, Universidad Autónoma de Madrid, Madrid, SpainSearch for more papers by this author First published: 01 May 2007 https://doi.org/10.1562/0031-8655(2001)0730283PIBZIP2.0.CO2Citations: 4 ¶ Posted on the website on 30 November 2000. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat ABSTRACT We have studied the photosensitizing effects of zinc(II)-phthalocyanine (ZnPc) on the cytoskeleton of HeLa cells using sublethal (10−7M, followed by 1 or 3 min of red light to induce 20%, LD20, or 60%, LD60, cell death, respectively) or lethal (5 × 10−6M and 15 min of irradiation, LD100) experimental conditions. The immunofluorescent analysis of the cytoskeleton showed a variable photodamage to microtubules (MT), actin microfilaments (AF) and intermediate filaments of keratin (KF), as well as on α-actinin, which was dependent on treatment conditions. Both sublethal treatments induced deep alterations on interphase and mitotic MT. The mitotic index increased with time with the maximum at 18 h (12%) or 24 h (14%) after LD20 or LD60, respectively. The alterations on AF and α-actinin were much more severe than those observed on KF at any evaluated time. With the exception of the KF, which remained partially organized, the MT and AF network was severely damaged by the lethal treatment. Western blot analysis for α-tubulin, G-actin and α-actinin from soluble and insoluble fractions confirmed the results observed by immunofluorescence, thus indicating that these cytoskeletal components are involved in cell damage and death by ZnPc photosensitization. Citing Literature Volume73, Issue3March 2001Pages 283-289 RelatedInformation