Origin and Development of Pollen Embryoids and Pollen Calluses in Cultured Anther Segments of Hyoscyamus niger (Henbane)
1978; Wiley; Volume: 65; Issue: 9 Linguagem: Inglês
10.2307/2442686
ISSN1537-2197
Autores Tópico(s)African Botany and Ecology Studies
ResumoAmerican Journal of BotanyVolume 65, Issue 9 p. 984-1002 Article ORIGIN AND DEVELOPMENT OF POLLEN EMBRYOIDS AND POLLEN CALLUSES IN CULTURED ANTHER SEGMENTS OF HYOSCYAMUS NIGER (HENBANE) V. Raghavan, V. Raghavan Department of Botany, The Ohio State University, Columbus, 43210Search for more papers by this author V. Raghavan, V. Raghavan Department of Botany, The Ohio State University, Columbus, 43210Search for more papers by this author First published: 01 October 1978 https://doi.org/10.1002/j.1537-2197.1978.tb06164.xCitations: 27AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The dedifferentiation of pollen grains of Hyoscyamus niger (henbane) into embryoids and calluses was examined by culturing identical segments of the same anther in a mineral salt-sucrose basal medium and in the basal medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid, respectively. Addition of auxin enhanced anther efficiency but did not affect the number of embryogenic pollen grains of an anther segment transformed into calluses. In anther segments cultured in the basal medium, the organogenetic part of the pollen embryoid was formed by the division of the generative cell alone, or by the division of both generative and vegetative cells. More or less similar pathways were followed by pollen grains of anther segments cultured in a medium containing auxin to form calluses. Culture of anther segments in a medium containing a high concentration of auxin (50.0 mg/l) led to a significant reduction in the yield of calluses which were formed almost entirely by the division of both generative and vegetative cells. The bearing of these observations on the role of auxin in determining the pathway of differentiation of embryogenic pollen grains in cultured anther segments is considered. The appearance of embryogenic pollen grains in close proximity to the tapetum as seen in longitudinal sections of cultured anther segments has suggested a role for a gradient of tapetal factors in embryogenic induction. Citing Literature Volume65, Issue9October 1978Pages 984-1002 RelatedInformation
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