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Correction: Label-Free Proteomics Reveals Decreased Expression of CD18 and AKNA in Peripheral CD4 + T Cells from Patients with Vogt-Koyanagi-Harada Syndrome

2011; Public Library of Science; Volume: 6; Issue: 3 Linguagem: Inglês

10.1371/annotation/2c8e3e74-556c-4e42-a5df-0b8ce294119f

1932-6203

Liming Mao, Peizeng Yang, Shengping Hou, Fuzhen Li, Aize Kijlstra,

Ocular Diseases and Behçet’s Syndrome

Vogt-Koyanagi-Harada (VKH) syndrome is a systemic autoimmune disease.CD4 + T cells have been shown to be involved in autoimmune diseases including VKH syndrome.To screen aberrantly expressed membrane proteins in CD4 + T cell from patients with active VKH syndrome, blood samples were taken from five patients with active VKH syndrome and five healthy individuals.A label-free quantitative proteomic strategy was used to identify the differently expressed proteins between the two groups.The results revealed that the expression of 102 peptides was significantly altered (p,0.05) between two groups and matched amino acid sequences of proteins deposited in the international protein index (ipi.HUMAN.v3.36.fasta).The identified peptides corresponded to 64 proteins, in which 30 showed more than a 1.5-fold difference between the two groups.The decreased expression of CD18 and AKNA transcription factor (AKNA), both being three-fold lower than controls in expression identified by the label-free method, was further confirmed in an additional group of five active VKH patients and six normal individuals using the Western blot technique.A significantly decreased expression of CD18 and AKNA suggests a role for both proteins in the pathogenesis of this syndrome.