Response of the amphibian tadpole Xenopus laevis to atrazine during sexual differentiation of the ovary
2002; Wiley; Volume: 21; Issue: 6 Linguagem: Inglês
10.1002/etc.5620210621
ISSN1552-8618
AutoresLuz E. Tavera-Mendoza, Sylvia M. Ruby, Pauline Brousseau, Michel Fournier, Daniel G. Cyr, David J. Marcogliese,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoAbstract Xenopus laevis tadpoles (stage 56) were exposed to 21 μg/L atrazine under laboratory‐controlled conditions in a static system. Following a 48‐h exposure period at 21 ± 0.5°C during sexual differentiation, tadpoles were fixed, and the kidney‐gonad complex was microdissected. Quantitative histological analysis revealed in a trazine‐exposed ovaries a significant ( p < 0.05) increase in frequency of secondary oogonia. Atresia, or oogonial resorption of both primary and secondary oogonia, also increased significantly ( p < 0.05). The results suggest that these primary germ cells, which constitute the total number of germ cells in the ovary for the reproductive life of the organism, were reduced by 20% following a 48‐h exposure period compared to 2% in controls.
Referência(s)