Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain
1999; Wiley; Volume: 64; Issue: 6 Linguagem: Inglês
10.1002/(sici)1097-0290(19990920)64
ISSN1097-0290
AutoresLucı́lia Domingues, Maria M. Dantas, Nelson Lima, J. A. Teixeira,
Tópico(s)Fermentation and Sensory Analysis
ResumoAlcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for -galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus.Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented.In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value.For the continuously operating bioreactor, an ethanol productivity of 11 g L -1 h -1 (corresponding to a feed lactose concentration of 50 g L -1 and a dilution rate of 0.55 h -1 ) was obtained, which is 7 times larger than the continuous conventional systems.The system stability was confirmed by keeping it in operation for 6 months.
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