Acetolysis of Leuconostoc mesenteroides NRRL B-1299 Dextran. Isolation and characterization of tetrasacharrides containing secondary linkages from the borate-insoluble fraction
1978; Elsevier BV; Volume: 61; Issue: 1 Linguagem: Inglês
10.1016/s0008-6215(00)84472-9
ISSN1873-426X
AutoresToshiyuki Watanabe, Ken-ichi Shishido, Mikihiko Kobayashi, Kazuo Matsuda,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoFractionation of the deacetylated acetolyzate of the borate-insoluble fraction of the dextran elaborated by Leuconostoc mesenteroides NRRL B-1299 gave five tetrasaccharide fractions, isolated after chromatography on charcoal—Celite, paper chromatography, and paper electrophoresis. Examination of partial acid hydrolyzates of the tetrasaccharide fractions and their corresponding alditols, the relation between the logarithm of their partition functions (α') and molecular size, and methylation studies, showed them to be (a) 23-α-d-glucosyl-nigerotriose (1), (b) a mixture of 6-α-nigerotriosyl-d-glucose (2) and 61-α-d-glucosyl-nigerotriose (3) and/or 62-α-d-glucosyl-nigerotriose (4), (c) a mixture of 21-α-nigerosyl-isomaltose (5) and 32-α-isomaltosyl-kojibiose (6) and/or 62-α-nigerosyl-kojibiose (7), (d) 2-α-nigerotriosyl-d-glucose (8) and (e) nigerotetraose (9).
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