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Ultrastructural Aspects of Shoot Initiation in Tobacco Callus Cultures

1973; Wiley; Volume: 60; Issue: 8 Linguagem: Inglês

10.2307/2441171

1537-2197

Malcolm K. Ross, Trevor A. Thorpe, J. W. Costerton,

Plant Disease Resistance and Genetics

American Journal of BotanyVolume 60, Issue 8 p. 788-795 Article ULTRASTRUCTURAL ASPECTS OF SHOOT INITIATION IN TOBACCO CALLUS CULTURES Malcolm K. Ross, Malcolm K. Ross Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this authorTrevor A. Thorpe, Trevor A. Thorpe Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this authorJ. William Costerton, J. William Costerton Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this author Malcolm K. Ross, Malcolm K. Ross Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this authorTrevor A. Thorpe, Trevor A. Thorpe Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this authorJ. William Costerton, J. William Costerton Department of Biology, University of Calgary, Calgary, Alberta, Canada, T2N 1N4Search for more papers by this author First published: 01 September 1973 https://doi.org/10.1002/j.1537-2197.1973.tb07590.xCitations: 30AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract An ultrastructural investigation of shoot initiation in tobacco (Nicotiana tabacum L. var. W. 38) callus cultures was made. Zones of preferential division were observed in the basal portion of the tissue by eight days in culture and these led, sequentially, to meristemoids, primordia, and shoots. During the initial stages of meristemoid formation, protein inclusions and large accumulations of plastid starch were present in the cells, while vacuoles were filled with membranous and cytoplasmic protrusions. At later stages of meristemoid development, these features were not observed in the cells, which were also smaller in size and possessed numerous small, peripheral vacuoles. It appears that the membranous and cytoplasmic protrusions are involved in vacuolar reduction during meristemoid formation. It would also appear that the storage materials supply the energy and other reserves needed for the organogenetic process. By contrast, tissue cultured under nonshoot-forming conditions and nonmeristemoid regions of shoot-forming tissue remained parenchymatous over the same time period. Citing Literature Volume60, Issue8September 1973Pages 788-795 RelatedInformation