Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA
1991; Oxford University Press; Volume: 82; Issue: 2 Linguagem: Inglês
10.1111/j.1574-6968.1991.tb04868.x
ISSN1574-6968
AutoresDaniel Simon, Joseph J. Ferretti,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoElectrotransformation was used to introduce both plasmid and linear DNA into Streptococcus pyogenes. The method was optimized using strain NZ131, for which transformation frequencies up to 107 per μg of plasmid DNA were obtained. A linear fragment of DNA, containing the streptokinase gene (ska) in which an internal fragment had been replaced with an erythromycin resistance gene (erm), was transformed into strain NZ131 with a frequency of 103 per μg DNA. The introduction of linear DNA into S. pyogenes by electrotransformation should be useful for future genetic analyses as well as targeted gene replacement.
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