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Protecting effect of Enterococcus faecium on intestinal epithelial structure in Balb/c mice immunized via intra-peritoneal way by β-lactoglobulin

2014; Frontiers Media; Volume: 5; Linguagem: Inglês

10.3389/conf.fimmu.2014.04.00023

1664-3224

DIB Wafaa, BISCOLA Vanessa, GOURINE Hanane, GRAR Hadria, CHOISET Yvan, CHOBERT Jean-Marc, HAERTLE Thomas, CHEKROUN Abdallah, SAIDI Djamel, KHEROUA Omar,

Probiotics and Fermented Foods

Event Abstract Back to Event Protecting effect of Enterococcus faecium on intestinal epithelial structure in Balb/c mice immunized via intra-peritoneal way by β-lactoglobulin Wafaa DIB1*, Vanessa BISCOLA2, Hanane GOURINE1, Hadria GRAR1, Yvan CHOISET3, Jean-Marc CHOBERT3, Thomas HAERTLE3, Abdallah CHEKROUN1, Djamel SAIDI1 and Omar KHEROUA1 1 Laboratory of Nutrition physiology and Food Safety, Biology Department, Faculty of Sciences, Oran University, Algeria 2 Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo.São Paulo, Brazil 3 UR 1268 Biopolymères, Interactions, Assemblage, équipe FIP, INRA, 44300 Nantes, France Introduction Probiotics have immuno-modulatory action by exerting stimulatory effects on the intestinal innate and adaptive immune system, enhancing mucosal barrier functions, inducing the production of anti-inflammatory cytokines, and facilitating the maintenance of immune tolerance. The effect of Enterococcus faecium on murine intestine is presented. Materials and methods 30 Balb/c mice were divided in three lots of 10 mice each. During an initial period of 18 days, the animals from the first and second lots received via an oral way a suspension of 0.3 ml of Enterococcus faecium WD15 and saline solution (positive control). Third lot (negative control) did not receive any treatment. Later, mice from the first and second lots were immunized via intra-peritoneal way using β-Lg. They were sacrificed on the 35th day and histological studies were conducted on their jejunum fragments. The score given to fragments of jejunum took into account the number of inflammations as well as their severity. The measurement of the length of the jejunum villi was performed using an optical microscope equipped with a micrometer. The level of IgG, IgG2a, IgG1 and IgE anti-β-Lg was determined in the sera by ELISA. The LAB strain was identified as Enterococcus faecium by 16S rDNA sequencing. Results and discussion Our results demonstrated that Enterococcus faecium WD15 has not modified the aspect and the structure of the villi, compared to the negative control group. Furthermore, the intra-epithelial lymphocytes infiltrations are little marked and length of villi in lot 1was 50.2 µm. These results agree with those of Dib et al. (2014a). In contrast, a partial atrophy (31.8 µm) (p≤ 0.0001), a marked inflammatory infiltration of lymphocytes in the chorion were observed in the positive control group, what altered the structure of the intestinal mucosa (score 4) as compared to the negative control group; similar data were reported by Addou et al. (2007). Enterococcus faecium markedly reduced the allergic response. It does not affect whey specific IgE and IgG1 responses, but IgG2a levels were increased; these results are in agreement with those of Dib et al. (2014b). Conclusion Presence of Enterococcus faecium WD15 protects mice against inflammatory responses in the gut and modulates the systemic immune response to β-lactoglobulin. Acknowledgements We would like to express our gratitude for the support of this work in the frame of TASSILI project #10MDU 792 “Alimentation de l’enfant allergique: Allergénicité des protéines du lait bovin après traitements technologiques et étude du rôle prophylactique des laits hypoallergéniques chez le model animal d’allergie et les enfants à risque” and for Algerian Ministry of higher Education and Scientific Research (DR-RSDT) for their financial support. References Addou-Benounane S, Tomé D, Kheroua O, Saidi D. Parenteral immunization to beta-lactoglobulin modifies the intestinal structure and mucosal electrical parameters in rabbit. Int Immunopharmacol (2004) 4: 1559-63. Dib W, Chekroun A, El-Ghaish S, Choiset Y, Chobert J-M, Haertlé T, Saidi D, Kheroua O. Effects of orally administered Enterococcus faecium on the immune systemic response and intestinal epithelial structure in balb/c mice immunized by bovine β-lactoglobulin. Int J Pharm Pharm Sci (2014a) 6: 587-90. Dib W, Rouane M, Gourine H, Grar H, Biscola V, Zouaoui S.F, Choiset Y, Chobert J-M, Haertlé T, Chekroun A, Saidi D, Kheroua O. Effet immunomodulateur d’Enterococcus sur la réponse allergénique à la β-lactoglobuline chez la souris Balb/c. REV FR ALLERGOL (2014b) 54: 276-77. Keywords: Beta-lactoglobulin, BALB/c, Enterococcus faecium WD15, IgG, IgE, intestinal epithelium, Lactic acid bacteria Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Oral Presentation Topic: Mucosal immunity Citation: DIB W, BISCOLA V, GOURINE H, GRAR H, CHOISET Y, CHOBERT J, HAERTLE T, CHEKROUN A, SAIDI D and KHEROUA O (2014). Protecting effect of Enterococcus faecium on intestinal epithelial structure in Balb/c mice immunized via intra-peritoneal way by β-lactoglobulin. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00023 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Miss. Wafaa DIB, Laboratory of Nutrition physiology and Food Safety, Biology Department, Faculty of Sciences, Oran University, Oran, Algeria, dibwafaa@hotmail.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Wafaa DIB Vanessa BISCOLA Hanane GOURINE Hadria GRAR Yvan CHOISET Jean-Marc CHOBERT Thomas HAERTLE Abdallah CHEKROUN Djamel SAIDI Omar KHEROUA Google Wafaa DIB Vanessa BISCOLA Hanane GOURINE Hadria GRAR Yvan CHOISET Jean-Marc CHOBERT Thomas HAERTLE Abdallah CHEKROUN Djamel SAIDI Omar KHEROUA Google Scholar Wafaa DIB Vanessa BISCOLA Hanane GOURINE Hadria GRAR Yvan CHOISET Jean-Marc CHOBERT Thomas HAERTLE Abdallah CHEKROUN Djamel SAIDI Omar KHEROUA PubMed Wafaa DIB Vanessa BISCOLA Hanane GOURINE Hadria GRAR Yvan CHOISET Jean-Marc CHOBERT Thomas HAERTLE Abdallah CHEKROUN Djamel SAIDI Omar KHEROUA Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.