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Solvent effects on the catalytic activity of subtilisin suspended in compressed gases

1996; Wiley; Volume: 49; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-0290(19960220)49

1097-0290

Inês Borges de Carvalho, Teresa Corrêa de Sampaio, Susana Barreiros,

Biofuel production and bioconversion

Biotechnology and BioengineeringVolume 49, Issue 4 p. 399-404 Article Solvent effects on the catalytic activity of subtilisin suspended in compressed gases Inês Borges de Carvalho, Inês Borges de Carvalho Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this authorTeresa Corrêa de Sampaio, Teresa Corrêa de Sampaio Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this authorSusana Barreiros, Corresponding Author Susana Barreiros Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalInstituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this author Inês Borges de Carvalho, Inês Borges de Carvalho Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this authorTeresa Corrêa de Sampaio, Teresa Corrêa de Sampaio Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this authorSusana Barreiros, Corresponding Author Susana Barreiros Instituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalInstituto de Tecnológia Química e Biológica, Universidade Nova de Lisboa, R. da Quinta Grande, 6, Apt. 127, 2780 Oeiras, PortugalSearch for more papers by this author First published: 20 February 1996 https://doi.org/10.1002/(SICI)1097-0290(19960220)49:4 3.0.CO;2-KCitations: 15AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract We studied a model transesterification reaction catalyzed by subtilisin Carlsberg suspended in carbon dioxide, propane, and mixtures of these solvents under pressure. To account for solvent effects due to differences in water partitioning between the enzyme and the bulk solvents, we measured water sorption isotherms for the enzyme in each solvent. We measured catalytic activity as a function of enzyme hydration and obtained bell-shaped curves with maxima at the same enzyme hydration (12%) in all the solvents. However, the activity maxima were different in all media, being much higher in propane than in either CO2 or the mixtures with 50 and 10% CO2. Considerations based on the solvation ability of the solvents did not offer an explanation for the differences in catalytic activity observed. 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