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Alterations of pulmonary capillary filtration and leukotriene synthesis due to infusion of a lipid emulsion enriched with Omega-3-fatty acids

1995; Taylor & Francis; Volume: 6; Issue: 3 Linguagem: Inglês

10.1080/714028885

1473-7752

T. Koch, A. Heller, I. Breil, K. Van Ackern, H. Neuhof,

Neuroscience of respiration and sleep

Objective: This study focuses on the question of whether the pulmonary response to inflammatory stimulation, resulting in increased vascular resistance and permeability, could be attenuated by short-term infusion of triglycerides containing omega (n)-3 fatty acids.The parenteral application of a lipid emulsion prepared from fish oil (Omegavenös®) was tested in comparison with a soy oil preparation (Lipovenös®).The hypothesis was that infusion of eicosapentaenoic acid (EPA), the major n-3 fatty acid contained in fish oil, may interfere with eicosanoid synthesis in such a manner that stimulation results in the release of the less vasoconstrictive and permeability enhancing metabolites of EPA instead of those of arachidonic acid (AA).Design: Isolated lungs from anaesthetised rabbits were ventilated and recirculation-perfused (200 ml/min) with 250 ml cell-free buffer solution to which either 2.5 ml saline (controls, n=3), 2.5 ml Lipovenös® 10% (n=11), or 2.5 ml Omegavenös® 10% (n=13) were added.To study the alterations of pulmonary vascular permeability in states of inflammatory stimulation, lungs of each group were stimulated with calcium-ionophore A23187 (1 µM).The capillary filtration coefficients (C fc ), as an index of permeability, were determined before and after administration of A23187 at 30-minute intervals during an observation period of 210 minutes.To study lipid effects on neutrophil-mediated inflammatory reactions, the same protocol was performed in additional experiments in which isolated human polymorphonuclear (PMN) leukocytes (10 8 ) were added to the perfusion fluid.For analysis of the 5-lipoxygenase mediator profile, perfusate samples were taken at 1-hour intervals.