Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats
2015; Wiley; Volume: 127; Issue: 49 Linguagem: Inglês
10.1002/ange.201507530
ISSN1521-3757
AutoresRyosuke Kojima, Hideo Takakura, Mako Kamiya, Eiji Kobayashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano,
Tópico(s)Electrochemical sensors and biosensors
ResumoAbstract A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO 3 H‐APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme‐induced electron transfer (BioLeT) and modulation of cell‐membrane permeability. This probe enables non‐invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes.
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