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Myrosinase Activity in Soil

2003; Wiley; Volume: 67; Issue: 1 Linguagem: Inglês

10.2136/sssaj2003.0139

1435-0661

Ahmad Alturki, Warren A. Dick,

Free Radicals and Antioxidants

Soil Science Society of America JournalVolume 67, Issue 1 p. 139-145 Division S-3—Soil Biology & Biochemistry Myrosinase Activity in Soil Ahmad I. Al-Turki, Ahmad I. Al-Turki King Saud University Qasim Branch, Buraidah Soil and Water Department, P.O. Box 1482, Saudi ArabiaSearch for more papers by this authorWarren A. Dick, Corresponding Author Warren A. Dick dick.5@osu.edu School of Natural Resources, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691-4096Corresponding author (dick.5@osu.edu)Search for more papers by this author Ahmad I. Al-Turki, Ahmad I. Al-Turki King Saud University Qasim Branch, Buraidah Soil and Water Department, P.O. Box 1482, Saudi ArabiaSearch for more papers by this authorWarren A. Dick, Corresponding Author Warren A. Dick dick.5@osu.edu School of Natural Resources, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691-4096Corresponding author (dick.5@osu.edu)Search for more papers by this author First published: 01 January 2003 https://doi.org/10.2136/sssaj2003.1390Citations: 32Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Myrosinase (thioglucoside glucohydrolase; EC 3.2.3.1) is an enzyme that hydrolyzes glucosinolates to D-glucose and allelochemicals that have biological potential to suppress weed seed germination in soil. This enzyme, found in some microorganisms and released to soils via root exudation and decomposition, can be assayed by adding sinigrin (2-propenyl-glucosinolate) to soil as a substrate. We describe a simple and rapid method to assay myrosinase activity in soils. In this method, 1 g of soil is treated with toluene (0.2 mL) and incubated at 37°C with 2.8 mL of a buffered solution (pH 7) of sinigrin (20 mM final concentration) for 4 h. Glucose released upon sinigrin hydrolysis is extracted and its concentration is measured spectrophotometrically. Tests showed that recovery of glucose was quantitative if toluene was included in the assay mixture. Myrosinase activity in five soils studied ranged from 71 to 338 μg glucose g−1 soil 4 h−1 The rate of sinigrin hydrolysis increased with temperature from 10 to 40°C. The activation energy of myrosinase in four soils ranged from 40.3 to 52.8 kJ mol−1 The Vmax values for sinigrin hydrolysis calculated by the three linear transformations of the Michaelis–Menten equation ranged from 76 to 518 (avg. 275) μg glucose g−1 soil 4 h−1 and the apparent Km values for myrosinase ranged from 5.3 to 12.9 (avg. 8.1) mM The method developed in this study is accurate, fast, and simple. Citing Literature Volume67, Issue1January 2003Pages 139-145 RelatedInformation