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Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

2015; Volume: 72; Issue: 2 Linguagem: Inglês

10.5505/turkhijyen.2015.88393

1308-2523

Feyza Çetin, Alparslan Toyran, Özlem Aytaç, Feride Alaca Coşkun, İpek Mumcuoğlu, Feyza Alp, Altan Aksoy,

RNA Interference and Gene Delivery

Objective: Systemic lupus erythematosus (SLE) is an autoimmune disease caused by antibodies which produced against antigens commonly on cell nuclei.According to the criteria of American College of Rheumatology (ACR), the immunological parameters which are used for diagnosis of SLE, are anti-nuclear antibodies (ANA) and anti-dsDNA autoantibodies.In this study it is aimed to investigate the specifity of three different ELISA tests by comparing with CLIF test, as a reference method, for the determination of anti-dsDNA antibodies.Methods: Sera of 81 patients who were diagnosed as SLE and sent to the Microbiology Department of our hospital between 1 May -31 July 2013, were included in the study.In these sera, ANA positivity was firstly investigated by immunofluorescence antibody test (IFA).Positive sera were stored at -80 O C for prospective analysis and processed with four different anti-dsDNA assays at the same day.These assays were three anti-dsDNA ELISA kits including; CHORUS dsDNA-G (DIESSE Diagnostica Senese, Italy), anti-dsDNA-Ncx ELISA IgG (EUROIMMUN, Germany, California), QUANTA Lite dsDNA SC ELISA (INOVA Diagnostics) and CLIF test (Crithidia luciliae anti-dsDNA, EUROIMMUN, Germany).Results: ANA patterns of patients defined by IFA were determined as; 35.8% homogeneous pattern, 22.2% homogeneous and other patterns, 24.7% granular pattern, 8.7% granular and other patterns, 7.4% nucleolar pattern and 1.2% centromere pattern. ÖZET Amaç:Sistemik lupus eritematozus (SLE) hücre çekirdeğindeki antijenlere karşı antikorların oluşturduğu otoimmün bir hastalıktır.Amerikan Romatoloji Derneği (ACR) kriterlerine göre SLE tanısında kullanılan immünolojik parametreler anti nükleer antikor (ANA) ve anti-dsDNA otoantikorlarıdır.Bu çalışmada anti-dsDNA antikorların saptanmasında, CLIF yöntemi referans yöntem kabul edilerek, üç farklı ELISA kitinin özgüllüğünün araştırılması amaçlanmıştır.Yöntemler: Çalışmaya 1 Mayıs -31 Temmuz 2013 tarihleri arasında hastanemiz mikrobiyoloji laboratuvarına gönderilen klinik olarak SLE tanısı almış olan 81 hastanın serum örnekleri dahil edilmiştir.Bu serumlarda ANA varlığı