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The Application of a Compact Multispectral Imaging System with Integrated Excitation Source to In vivo Monitoring of Fluorescence During Topical Photodynamic Therapy of Superficial Skin Cancers¶

2001; Wiley; Volume: 73; Issue: 3 Linguagem: Inglês

10.1562/0031-8655(2001)073 2.0.co;2

1751-1097

Jacqueline Hewett, Valerie Nadeau, James Ferguson, Harry Moseley, Sally H. Ibbotson, John W. Allen, Wilson Sibbett, Miles J. Padgett,

Photoacoustic and Ultrasonic Imaging

Photochemistry and PhotobiologyVolume 73, Issue 3 p. 278-282 The Application of a Compact Multispectral Imaging System with Integrated Excitation Source to In vivo Monitoring of Fluorescence During Topical Photodynamic Therapy of Superficial Skin Cancers¶ Jacqueline Hewett, Corresponding Author Jacqueline Hewett School of Physics and Astronomy, University of St. Andrews, St. Andrews, UK *To whom correspondence should be addressed at: School of Physics and Astronomy, University of St. Andrews, St. Andrews KY16 9SS, UK. Fax: 44-1334-463104;[email protected]Search for more papers by this authorValerie Nadeau, Valerie Nadeau Department of Physics and Astronomy, University of Glasgow, Glasgow, UKSearch for more papers by this authorJames Ferguson, James Ferguson Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorHarry Moseley, Harry Moseley Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorSally Ibbotson, Sally Ibbotson Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorJohn W. Allen, John W. Allen School of Physics and Astronomy, University of St. Andrews, St. Andrews, UKSearch for more papers by this authorWilson Sibbett, Wilson Sibbett School of Physics and Astronomy, University of St. Andrews, St. Andrews, UKSearch for more papers by this authorMiles Padgett, Miles Padgett Department of Physics and Astronomy, University of Glasgow, Glasgow, UKSearch for more papers by this author Jacqueline Hewett, Corresponding Author Jacqueline Hewett School of Physics and Astronomy, University of St. Andrews, St. Andrews, UK *To whom correspondence should be addressed at: School of Physics and Astronomy, University of St. Andrews, St. Andrews KY16 9SS, UK. Fax: 44-1334-463104;[email protected]Search for more papers by this authorValerie Nadeau, Valerie Nadeau Department of Physics and Astronomy, University of Glasgow, Glasgow, UKSearch for more papers by this authorJames Ferguson, James Ferguson Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorHarry Moseley, Harry Moseley Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorSally Ibbotson, Sally Ibbotson Photobiology Unit, Department of Dermatology, Ninewells Hospital and Medical School, University of Dundee, Dundee, UKSearch for more papers by this authorJohn W. Allen, John W. Allen School of Physics and Astronomy, University of St. Andrews, St. Andrews, UKSearch for more papers by this authorWilson Sibbett, Wilson Sibbett School of Physics and Astronomy, University of St. Andrews, St. Andrews, UKSearch for more papers by this authorMiles Padgett, Miles Padgett Department of Physics and Astronomy, University of Glasgow, Glasgow, UKSearch for more papers by this author First published: 01 May 2007 https://doi.org/10.1562/0031-8655(2001)0730278TAOACM2.0.CO2Citations: 6 ¶ Posted on the website on 8 January 2001. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat ABSTRACT A novel, compact and low-cost multispectral fluorescence imaging system with an integrated excitation light source is described. Data are presented demonstrating the application of this method to in vivo monitoring of fluorescence before, during and after topical 5-aminolevulinic acid photodynamic therapy of superficial skin cancers. The excitation source comprised a fluorescent tube with the phosphor selected to emit broadband violet light centered at 394 nm. The camera system simultaneously captured spectrally specific images of the fluorescence of the photosensitizer, protoporphyrin IX, the illumination profile and the skin autofluorescence. Real-time processing enabled images to be manipulated to create a composite image of high contrast. The application and validation of this method will allow further detailed studies of the characteristics and time-course of protoporphyrin IX fluorescence, during topical photodynamic therapy in human skin in vivo. Citing Literature Volume73, Issue3March 2001Pages 278-282 RelatedInformation