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Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars

2009; Elsevier BV; Volume: 12; Issue: 3 Linguagem: Inglês

10.2225/vol12-issue3-full1

0717-3458

Ghulam Muhammad Ali, Umer Rashid, Shaukat Ali, Najma Ayub, M. Shahid Masood,

Plant Pathogens and Resistance

6-γ-γ-dimethylallylaminopurine BAP: 6-BenzylAminoPurine IAA: Indole-3-Acetic Acid Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation.For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated.For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested.Best hormone combinations were further subjected to Kinetin and 6-γ-γ-dimethylallylaminopurine (2iP).For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25%, 77.75% and 95.20% of embryogenic calli, respectively.Maximum callus induction (97.18%) was observed in Tatara when 2 mg/L of 2,4-D was used.As regard to regeneration, Inqilab-91, Chakwal-97 and Manthar showed maximum regeneration on media containing 0.1 mg/L IAA, 0.4 mg/L Kinetin and 0.5 mg/L 2iP,