Creatine Kinase
1974; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-091302-2.50056-6
AutoresGeorg Forster, Erich Bernt, Hans Ulrich Bergmeyer,
Tópico(s)Cardiomyopathy and Myosin Studies
ResumoThis chapter focuses on creatine kinase (CK), which is mainly found in the cytoplasm of the cell. CK is found in order of decreasing activity in the following human organs: skeletal muscle, heart muscle, cerebral cortex, smooth muscle, thyroid glands, kidne, and liver. In clinical studies on the activity in serum using the method of Tanzer and Gilvarg, CK was found to be unstable. CK is applied in biochemistry and in clinical chemistry for the diagnosis of myocardial infarction and of myopathies, in particular progressive muscular dystrophy and dermatomyositis. The assay of SH-reactivated CK (addition of GSH) in serum has the advantage in the diagnosis of myocardial infarction that it not only shows a steep rise in activity to higher absolute values, but also the increase is maintained for 5 to 6 days. The pH optimum for the phosphorylation of creatine is pH 9.0 at 30°C. All solutions should be stoppered and stored in a refrigerator at 0–4°C. CK reacts specifically with creatine phosphate.
Referência(s)