Portal de Periódicos da CAPES

Three‐dimensional extracellular matrix engineering in the nervous system

1998; Wiley; Volume: 40; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-4636(19980603)40

1097-4636

Markus Borkenhagen, J.‐F. Clémence, Hans Sigrist, Patrick Aebischer,

Neuropeptides and Animal Physiology

Journal of Biomedical Materials ResearchVolume 40, Issue 3 p. 392-400 Three-dimensional extracellular matrix engineering in the nervous system M. Borkenhagen, M. Borkenhagen Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this authorJ.-F. Clémence, J.-F. Clémence Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this authorH. Sigrist, H. Sigrist CSEM, Centre Suisse d'Electronique et de Microtechnique SA, Neuchâtel, SwitzerlandSearch for more papers by this authorP. Aebischer, Corresponding Author P. Aebischer Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandDivision of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this author M. Borkenhagen, M. Borkenhagen Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this authorJ.-F. Clémence, J.-F. Clémence Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this authorH. Sigrist, H. Sigrist CSEM, Centre Suisse d'Electronique et de Microtechnique SA, Neuchâtel, SwitzerlandSearch for more papers by this authorP. Aebischer, Corresponding Author P. Aebischer Division of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandDivision of Surgical Research and Gene Therapy Center, Pavillon 4, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, SwitzerlandSearch for more papers by this author First published: 06 December 1998 https://doi.org/10.1002/(SICI)1097-4636(19980603)40:3 3.0.CO;2-CCitations: 126AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Growing neurites are guided through their environment during development and regeneration via different cellular and extracellular matrix (ECM) molecular cues. To mimic cell–matrix interactions, a three-dimensional (3D) hydrogel-based ECM equivalent containing a covalently immobilized laminin oligopeptide sequence was designed to facilitate nerve regeneration. This study illustrates that the oligopeptide domain CDPGYIGSR covalently linked to an agarose gel as a bioartificial 3D substrate successfully supports neurite outgrowth from dorsal root ganglia (DRG) in vitro. The specificity of the neurite promoting activity was illustrated through the inhibition of neurite outgrowth from DRG in a CDPGYIGSR-derivatized gel in the presence of solubilized CDPGYIGSR peptide. Gels derivatized with CDPGYIGSK and CDPGRGSYI peptides stimulated a smaller increase of neurite outgrowth. In vivo experiments revealed the capability of a CDPGYIGSR-derivatized gel to enhance nerve regeneration in a transected rat dorsal root model compared to an underivatized gel, a CDPGRGSYI gel, and saline-filled nerve guidance channels. These data suggest the feasibility of a 3D hydrogel-based ECM equivalent capable of enhancing neurite outgrowth in vitro and in vivo. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 40, 392–400, 1998. Citing Literature Volume40, Issue35 June 1998Pages 392-400 RelatedInformation