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The Mode of Cell Death Induced by Photodynamic Treatment Depends on Cell Density

1999; Wiley; Volume: 70; Issue: 3 Linguagem: Inglês

10.1562/0031-8655(1999)070 2.3.co;2

1751-1097

Jostein Dahle, Harald B. Steen, Johan Moan,

Photoreceptor and optogenetics research

Photochemistry and PhotobiologyVolume 70, Issue 3 p. 363-367 The Mode of Cell Death Induced by Photodynamic Treatment Depends on Cell Density Jostein Dahle, Corresponding Author Jostein Dahle Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, Norway*Department of Biophysics, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway. Fax: +47 22 93 42 70; e-mail:jostein.dahle@ labmed.uio.noSearch for more papers by this authorHarald B. Steen, Harald B. Steen Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, NorwaySearch for more papers by this authorJohan Moan, Johan Moan Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, NorwaySearch for more papers by this author Jostein Dahle, Corresponding Author Jostein Dahle Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, Norway*Department of Biophysics, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway. Fax: +47 22 93 42 70; e-mail:jostein.dahle@ labmed.uio.noSearch for more papers by this authorHarald B. Steen, Harald B. Steen Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, NorwaySearch for more papers by this authorJohan Moan, Johan Moan Department of Biophysics, The Norwegian Radium Hospital, Montebello, Oslo, NorwaySearch for more papers by this author First published: 02 January 2008 https://doi.org/10.1111/j.1751-1097.1999.tb08150.xCitations: 53AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Abstract Madison Darby canine kidney II (MDCK II) cells were seeded out at two different densities and incubated with 125 μ/mL of the photosensitizer me50-tetra(4-sulfona-tophenyUporphine (TPPS4) for 18 h, washed and irradiated with blue light. Four hours later the cells were studied by fluorescence microscopy. Apoptotic cells were detected by virtue of the distinct condensation and fragmentation of chromatin, and necrotic cells were detected by uptake of propidium iodide. In addition apoptosis was measured by the TdT assay. The fraction of apoptotic cells and the fraction of necrotic cells were determined for both cell densities at various levels of survival. With <55% total cell death the apoptotic fraction was significantly higher for cells in confluent monolayers than for cells growing in microcolonies at equitoxic doses. Confluent cells were 2.9 times more sensitive than cells in microcolonies partly due to a 1.5 times higher uptake of TPPS4 in monolayer cells. The difference in mode of cell death for the different cell densities was not related to any observable difference in subcellular localization pattern of TPPS4 at equitoxic doses of photodynamic treatment. Citing Literature Volume70, Issue3September 1999Pages 363-367 RelatedInformation