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Intracellular Inducible Alkylation System That Exhibits Antisense Effects with Greater Potency and Selectivity than the Natural Oligonucleotide

2006; Wiley; Volume: 118; Issue: 19 Linguagem: Inglês

10.1002/ange.200504441

1521-3757

Md. Monsur Ali, Motoi Oishi, Fumi Nagatsugi, Kenya Mori, Yukio Nagasaki, Kazunori Kataoka, Shigeki Sasaki,

Advanced biosensing and bioanalysis techniques

Eine Fehlpaarung in der mRNA-Zielsequenz wird durch ein System zur induzierbaren Alkylierung erkannt, das auf Sulfidvorstufen des Nucleosids 2-Amino-6-vinylpurin beruht (siehe Schema). Die reaktiven Oligonucleotide werden als Polyethylenglycol(PEG)-Konjugate in Polyionkomplex(PIC)-Micellen in die Zellen geschleust. Ihre Antisense-Aktivität ist hoch selektiv und übertrifft diejenige natürlicher Antisense-Oligonucleotide. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2006/z504441_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.