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Comparison of inflammatory cell counts in asthma: induced sputum vs bronchoalveolar lavage and bronchial biopsies

1997; Wiley; Volume: 27; Issue: 7 Linguagem: Inglês

10.1111/j.1365-2222.1997.tb01211.x

1365-2222

Diana C. Grootendorst, Jacob K. Sont, Luc Willems, Hanneke C. Kluin‐Nelemans, J.H.J.M. van Krieken, Maud Veselic‐Charvat, Peter Sterk,

Chronic Obstructive Pulmonary Disease (COPD) Research

Clinical & Experimental AllergyVolume 27, Issue 7 p. 769-779 Comparison of inflammatory cell counts in asthma: induced sputum vs bronchoalveolar lavage and bronchial biopsies D. C. GROOTENDORST, D. C. GROOTENDORST Departments of PulmonologySearch for more papers by this authorJ. K. SONT, J. K. SONT Departments of PulmonologySearch for more papers by this authorL. N. A. WILLEMS, L. N. A. WILLEMS Departments of PulmonologySearch for more papers by this authorJ. C. KLUIN-NELEMANS, J. C. KLUIN-NELEMANS Haematology, Laboratory of Haematomorphology and ImmunotypingSearch for more papers by this authorJ. H. J. M. VAN KRIEKEN, J. H. J. M. VAN KRIEKEN Pathology, Leiden University Medical Centre, The NetherlandsSearch for more papers by this authorM. VESELIC-CHARVAT, M. VESELIC-CHARVAT Pathology, Leiden University Medical Centre, The NetherlandsSearch for more papers by this authorP. J. STERK, Corresponding Author P. J. STERK Departments of PulmonologyProfessor P. J. Sterk, Lung Function Laboratory, C2-P, Department of Pulmonology, Leiden University Medical Centre, PO Box 9600, NL-2300 RC Leiden, The Netherlands.Search for more papers by this author D. C. GROOTENDORST, D. C. GROOTENDORST Departments of PulmonologySearch for more papers by this authorJ. K. SONT, J. K. SONT Departments of PulmonologySearch for more papers by this authorL. N. A. WILLEMS, L. N. A. WILLEMS Departments of PulmonologySearch for more papers by this authorJ. C. KLUIN-NELEMANS, J. C. KLUIN-NELEMANS Haematology, Laboratory of Haematomorphology and ImmunotypingSearch for more papers by this authorJ. H. J. M. VAN KRIEKEN, J. H. J. M. VAN KRIEKEN Pathology, Leiden University Medical Centre, The NetherlandsSearch for more papers by this authorM. VESELIC-CHARVAT, M. VESELIC-CHARVAT Pathology, Leiden University Medical Centre, The NetherlandsSearch for more papers by this authorP. J. STERK, Corresponding Author P. J. STERK Departments of PulmonologyProfessor P. J. Sterk, Lung Function Laboratory, C2-P, Department of Pulmonology, Leiden University Medical Centre, PO Box 9600, NL-2300 RC Leiden, The Netherlands.Search for more papers by this author First published: 28 June 2008 https://doi.org/10.1046/j.1365-2222.1997.890900.xCitations: 101 Supported by a grant from the Commission of the European Communities (ECSC grant 7280–03–056). AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Summary Background Induced sputum potentially allows monitoring of airway inflammation in patients with asthma in a non-invasive way. However, the relationship between the cellular content in sputum and airway tissue has not been fully clarified. Objective We compared the cellular compositions of hypertonic saline-induced sputum, bronchoalveolar lavage fluid (BAL) and bronchial biopsies in 18 clinically stable patients with mild to moderate atopic asthma (baseline FEV1: range 61–114%pred, PC20 methacholine: 0.04–4.7 mg/mL). They were treated with inhaled short-acting bronchodilators on demand, with (n = 8) or without (n= 10) regular inhaled steroids. Methods Each patient underwent sputum induction and fiberoptic bronchoscopy on separate days in random order. Differential cell counts of induced sputum, bronchoalveolar lavage and bronchial wash were determined on May-Grünwald-Giemsa stained cytospins. Flow cytometry was performed on sputum and BAL samples. Immunohistochemical techniques were used to stain inflammatory cells in 6 μm cryostat sections of bronchial biopsies. Results Sputum cell differentials were not different between the patients with and without inhaled steroids, and showed a median value of 19.4% squamous cells, with 1.0% eosinophils, 3.3% lymphocytes, 28.7% neutrophils, 49.4% macrophages and 6.9% cylindric epithelial cells (in percentage non-squamous cells). The percentage eosinophils in sputum was significantly correlated with their percentage in bronchial wash(Rs= 0.52, P = 0.03) and in BAL (Rs= 0.55,P = 0.02), whilst there was a trend towards such a correlation between the number of eosinophils/mL sputum and the number of EG2+ eosinophils/mm2 lamina propria in bronchial biopsies (Rs= 0.44,P= 0.07). In addition, the percentage of CD4+ lymphocytes correlated between sputum and BAL (Rs= 0.55, P= 0.03). Conclusion We conclude that the eosinophil counts in hypertonic saline-induced sputum from patients with asthma are related to those in bronchial wash and BAL and, to a lesser extent, with the counts in bronchial biopsies. This suggests that induced sputum can be used to monitor the presence and severity of airway inflammation in asthma. Citing Literature Volume27, Issue7July 1997Pages 769-779 RelatedInformation