Portal de Periódicos da CAPES

Plenary lectures

2018; De Gruyter; Volume: 41; Issue: s1 Linguagem: Inglês

10.2478/ctb-2018-0003

2084-1892

Stephan Uphoff, Mathew Stracy, Adam J. M. Wollman, Elżbieta Kaja, Jacek Gapiński, Tony Maxwell, David J. Sherratt, Mark Leak, Paweł Zawadzki, Krzysztof Wycisk, Aneta Tarczewska, Michał Taube, Maciej Kozak, Andrzej Ożyhar, Paulina Piotrowska, Magdalena Łazicka, Łucja Kowalewska, Joanna Grzyb, Magdalena Maj- Żurawska, Maciej Garstka, Kinga Matuła, Łukasz Richter, Marta Janczuk- Richter, Wojciech Nogala, Mikołaj Grzeszkowiak, Barbara Peplińska, Stefan Jurga, Elżbieta Wyroba, Szymon Suski, Henryk Bilski, Adrian Silesian, Hans A.R. Bluyssen, Natalia Derebecka, Joanna Wesoły, Joanna M. Łoś, Marcin Łoś, Przemysław Decewicz, Łukasz Dziewit, Jan Paczesny, Robert Hołyst,

The accurate detection and repair of DNA damage is crucial for genome stability in all organisms.Despite extensive characterization of DNA repair pathways using genetics and biochemical assays, it remains unclear how repair proteins perform their function within the cellular environment and how different repair pathways are coordinated in the cell.I will present our developments of single-molecule imaging and microfluidics techniques to investigate the mechanisms of DNA repair and mutagenesis at the single-cell level.A key advantage of these methods is their ability to resolve biological heterogeneity and dynamic behaviour.By avoiding population averaging, we found that mutation rates are dynamically regulated by DNA damage responses.We show that stochastic fluctuations in the expression of DNA repair factors affect cell survival and modulate the rates of mutagenesis in single bacterial cells.