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Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

2008; Hindawi Publishing Corporation; Volume: 22; Issue: 5 Linguagem: Inglês

10.1155/2008/715905

1875-922X

Grace O’Maille, Eden P. Go, Linh Hoang, Elizabeth J. Want, Colin A. Smith, Paul E. O’Maille, Anders Nordström, Hirotoshi Morita, Chuan Qin, Wilasinee Uritboonthai, Junefredo V. Apon, Richard O. Moore, James M. Garrett, Gary Siuzdak,

Analytical Chemistry and Chromatography

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.