EFFECT OF SOIL CONTAMINANT EXTRACTION METHOD IN DETERMINING TOXICITY USING THE MICROTOX® ASSAY
2000; Wiley; Volume: 19; Issue: 2 Linguagem: Inglês
10.1897/1551-5028(2000)019 2.3.co;2
ISSN1552-8618
AutoresGail A. Harkey, Thomas M. Young,
Tópico(s)bioluminescence and chemiluminescence research
ResumoEnvironmental Toxicology and ChemistryVolume 19, Issue 2 p. 276-282 Environmental Chemistry Effect of soil contaminant extraction method in determining toxicity using the microtox® assay Gail A. Harkey, Corresponding Author Gail A. Harkey [email protected] Institute of Gas Technology, 1700 South Mount Prospect Road, Des Plaines, Illinois 60018, USAInstitute of Gas Technology, 1700 South Mount Prospect Road, Des Plaines, Illinois 60018, USASearch for more papers by this authorThomas M. Young, Thomas M. Young Department of Civil and Environmental Engineering, University of California, Davis, California 95616, USASearch for more papers by this author Gail A. Harkey, Corresponding Author Gail A. Harkey [email protected] Institute of Gas Technology, 1700 South Mount Prospect Road, Des Plaines, Illinois 60018, USAInstitute of Gas Technology, 1700 South Mount Prospect Road, Des Plaines, Illinois 60018, USASearch for more papers by this authorThomas M. Young, Thomas M. Young Department of Civil and Environmental Engineering, University of California, Davis, California 95616, USASearch for more papers by this author First published: 02 November 2009 https://doi.org/10.1002/etc.5620190205Citations: 26Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract This project examined the influence of different extraction methods onthe measured toxicityofcontaminatedsoils collected from manufactured gas plant (MGP) sites differing in soil composition and contaminant concentration. Aged soils from a number of MGP sites were extracted using a saline solution, supercritical fluid extraction (SFE), and Soxhlet extraction. Toxicity was assessed using two forms of Microtox® tests: acute aqueous tests on saline and SFE soil extracts and solid-phase tests (SPTs) on soil particles. Microtox SPTs were performed on soils before and after SFE to determine resulting toxicity reduction. Three hypotheses were tested: (1) Toxicity of soil extracts is related to contaminant concentrations of the extracts, (2) measured toxicity significantly decreases with less vigorous methods of extraction, and (3) supercritical fluid extractability correlates with measured toxicity. The EC50s for SPTs performed before and after SFE were not different for some soils but were significantly greater after extraction for other soils tested. The most significant toxicity reductions were observed for soils exhibiting the highest toxicity in both preextraction SPTs and acute aqueous tests. Acute Microtox tests performed on SFE extracts showed significantly lower EC50s than those reported from saline-based extraction procedures. Toxicity of the soils measured by Microtox SPTs was strongly correlated with both SFE efficiency and measures of contaminant aging. Data from this project provide evidence of sequestration and reduced availability of polycyclic aromatic hydrocarbons (PAHs) from soils extracted via physiologically based procedures compared to vigorous physical extraction protocols. References 1 Scribner SL, Benzing TR, Boyd SA. 1992. Desorption and bioavailability of aged simazine residues in soil from a continuous corn field. J Environ Qual 21: 115–120. 2 Hatzinger PB, Alexander M. 1995. Effect of aging of chemicals in soil on their biodegradability and extractability. Environ Sci Technol 29: 537–545. 3 Pignatello JJ, Xing B. 1995. Mechanisms of slow sorption of organic chemicals to natural particles. Environ Sci Technol 30: 1–11. 4 Linz DG, Nakles DV. 1997. 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Citing Literature Volume19, Issue2February 2000Pages 276-282 ReferencesRelatedInformation
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