Portal de Periódicos da CAPES

SUBMITOCHONDRIAL PARTICLES AS TOXICITY BIOSENSORS OF CHLOROPHENOLS

1995; Wiley; Volume: 14; Issue: 3 Linguagem: Inglês

10.1897/1552-8618(1995)14[363

1552-8618

Emanuele Argese, Cinzia Bettiol, Anna Ghelli, Roberto Todeschini, P. Miana,

Electrochemical sensors and biosensors

Environmental Toxicology and ChemistryVolume 14, Issue 3 p. 363-368 Environmental Chemistry Submitochondrial particles as toxicity biosensors of chlorophenols Emanuele Argese, Corresponding Author Emanuele Argese Department of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalyDepartraent of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalySearch for more papers by this authorCinzia Bettiol, Cinzia Bettiol Department of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalySearch for more papers by this authorAnna Ghelli, Anna Ghelli Department of Biology, University of Bologna, Via Irneno, 42, 40126 Bologna, ItalySearch for more papers by this authorRoberto Todeschini, Roberto Todeschini Department of Physical Chemistry and Electrochemistry, University of Milano, Via Golgi 19, 20133 Milano, ItalySearch for more papers by this authorPaola Miana, Paola Miana ASPIV Venice Waterboard, S Croce 494, 30100 Venezia, ItalySearch for more papers by this author Emanuele Argese, Corresponding Author Emanuele Argese Department of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalyDepartraent of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalySearch for more papers by this authorCinzia Bettiol, Cinzia Bettiol Department of Environmental Sciences, University of Venezia, Calle Larga S Marta, 2137, 30123 Venezia, ItalySearch for more papers by this authorAnna Ghelli, Anna Ghelli Department of Biology, University of Bologna, Via Irneno, 42, 40126 Bologna, ItalySearch for more papers by this authorRoberto Todeschini, Roberto Todeschini Department of Physical Chemistry and Electrochemistry, University of Milano, Via Golgi 19, 20133 Milano, ItalySearch for more papers by this authorPaola Miana, Paola Miana ASPIV Venice Waterboard, S Croce 494, 30100 Venezia, ItalySearch for more papers by this author First published: March 1995 https://doi.org/10.1002/etc.5620140302Citations: 35AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract An in vitro bioassay procedure was used to investigate the toxic action of chlorophenols on mitochondrial respiratory parameters The toxicity of these compounds was evaluated by determining their effects on the energy coupled reverse electron transfer (RET) in submitochondrial particles (SMPs) from beef heart mitochondria The bioassay procedure is based on the spectrophotometric recording of the effects of toxicants on the rate of NAD+ reduction, induced by ATP and succinate at the first site level of the respiratory chain The toxicity end point was expressed as the toxicant concentration that causes 50% inhibition of NAD+ reduction rate (EC50). The EC50 values determined for the 14 tested chlorophenols ranged from 17 mg/L for 2 chlorophenol to 0 081 mg/L for pentachlorophenol, indicating a general trend of increasing toxicity with increasing chlorine substitution Among chlorophenol isomers, which have the same number of chlorine atoms, a lesser toxicity was associated with ortho-substituted chlorophenols, whereas meta substituted chlorophenols were much more toxic The EC50 values were compared with the toxicity data for a variety of bioassays, by means of linear regression analysis High degrees of correlation obtained with toxicity tests involving different freshwater species demonstrate the ability of SMPs to reproduce the toxic effects of the tested compounds upon aquatic organisms This supports the assessment that the respiratory chain is the main target of this class of toxicants Results obtained with chlorophenols and, in previous studies, with other environmental contaminants confirm the suitability of the SMP bioassay as a prescreening or complementary short term test for monitoring aquatic toxicity Citing Literature Volume14, Issue3March 1995Pages 363-368 RelatedInformation