Inhibition of IGFBP‐5 binding to extracellular matrix and IGF‐I–stimulated DNA synthesis by a peptide fragment of IGFBP‐5
1998; Wiley; Volume: 71; Issue: 3 Linguagem: Inglês
10.1002/(sici)1097-4644(19981201)71
ISSN1097-4644
Autores Tópico(s)Folate and B Vitamins Research
ResumoJournal of Cellular BiochemistryVolume 71, Issue 3 p. 375-381 Research Article Inhibition of IGFBP-5 binding to extracellular matrix and IGF-I–stimulated DNA synthesis by a peptide fragment of IGFBP-5 C. Rees, C. Rees Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599Search for more papers by this authorD.R. Clemmons, Corresponding Author D.R. Clemmons Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599Division of Endocrinology, CB #7170, 6111 Thurston Bowles Bldg., University of North Carolina, Chapel Hill, NC 27599Search for more papers by this author C. Rees, C. Rees Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599Search for more papers by this authorD.R. Clemmons, Corresponding Author D.R. Clemmons Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599Division of Endocrinology, CB #7170, 6111 Thurston Bowles Bldg., University of North Carolina, Chapel Hill, NC 27599Search for more papers by this author First published: 12 December 1998 https://doi.org/10.1002/(SICI)1097-4644(19981201)71:3 3.0.CO;2-MCitations: 16AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Insulin-like growth factor binding protein-5 (IGFBP-5) is synthesized and secreted by smooth muscle cells (SMC). IGFBP-5 synthesis is stimulated five- to sixfold by IGF-I, and IGFBP-5 has been shown to augment IGF-I–stimulated DNA synthesis in this cell type. The ability of IGFBP-5 to augment the SMC response to IGF-I is dependent upon its binding to extracellular matrix. A highly charged region of IGFBP-5 that contains amino acids in positions 201–218 has been shown to mediate binding of IGFBP-5 to human fibroblast extracellular matrix (ECM), and a synthetic peptide containing this sequence inhibits IGFBP-5 binding to fibroblast ECM. In this study we show that exposure of SMC cultures that are constituitively synthesizing IGFBP-5 to a synthetic peptide (termed peptide A) containing this sequence has no effect on its synthesis but reduces its abundance within the ECM. The addition of increasing concentrations of the peptide to SMC cultures resulted in a concentration-dependent reduction in ECM-associated IGFBP-5. In contrast, a control peptide (peptide B), which contained the region of amino acids in positions 131–141 and had a similar charge-to-mass ratio, caused a minimal decrease in ECM binding. This effect was functionally significant since the addition of 10 μg/ ml of peptide A inhibited the cellular replication response to 10 ng/ ml IGF-I by 51%, and peptide B had no effect. The effects of peptide A were not due to nonspecific cytotoxicity since it had no inhibitory effect on the response of these cells to human serum and was associated with only minimal inhibition of the cellular response to platelet-derived growth factor. The findings suggest that inhibiting IGFBP-5 binding to porcine SMC ECM results in reduced cellular responses to IGF-I. J. Cell. Biochem. 71:375–381, 1998. © 1998 Wiley-Liss, Inc. 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