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Genetic diversity of breeding popcorn lines determined by SSR markers

2010; Elsevier BV; Volume: 13; Issue: 1 Linguagem: Inglês

10.2225/vol13-issue1-fulltext-11

0717-3458

Carlos Alberto Scapim, Ana Paula Ribeiro Trindade, Ronald José Barth Pinto, Antônio Teixeira do Amaral Júnior, Claudete Aparecida Mangolin, María de Fátima Pires da Silva Machado,

Botanical Studies and Applications

Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations.Eight popcorn S 6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers.Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification.The genetic distance was estimated by Rogers' modified distance.The different popcorn breeding programs in Brazil are possibly using highly similar base-populations.The genetic similarity of lines *Corresponding author P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar.The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups.The clusters were consistent with the estimates of genetic identity.There was a moderate coincidence degree between the groups and genealogy of lines.Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4.Crosses between lines P1-3 and P8-1 are also promising.