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Comparative Measurements of Nuclear DNA in a Heterothallic and a Self-Fertile Isolate of the Myxomycete, Didymium iridis

1974; Wiley; Volume: 61; Issue: 4 Linguagem: Inglês

10.2307/2441807

1537-2197

C. Dale Therrien, John J. Yemma,

DNA and Biological Computing

American Journal of BotanyVolume 61, Issue 4 p. 400-404 Article COMPARATIVE MEASUREMENTS OF NUCLEAR DNA IN A HETEROTHALLIC AND A SELF-FERTILE ISOLATE OF THE MYXOMYCETE, DIDYMIUM IRIDIS C. D. Therrien, C. D. Therrien Department of Biology, Pennsylvania State University, University Park, 16802 Department of Biology, Youngstown State University, Youngstown, Ohio, 44503Search for more papers by this authorJohn J. Yemma, John J. Yemma Department of Biology, Pennsylvania State University, University Park, 16802 Department of Biology, Youngstown State University, Youngstown, Ohio, 44503Search for more papers by this author C. D. Therrien, C. D. Therrien Department of Biology, Pennsylvania State University, University Park, 16802 Department of Biology, Youngstown State University, Youngstown, Ohio, 44503Search for more papers by this authorJohn J. Yemma, John J. Yemma Department of Biology, Pennsylvania State University, University Park, 16802 Department of Biology, Youngstown State University, Youngstown, Ohio, 44503Search for more papers by this author First published: 01 April 1974 https://doi.org/10.1002/j.1537-2197.1974.tb12258.xCitations: 13AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Comparative measurements were made of the nuclear Feulgen-DNA content of a heterothallic and a self-fertile isolate of the myxomycete Didymium iridis. Plasmodial nuclei of both isolates contain the diploid amount of DNA. The replicated diploid (4C) values for the heterothallic and the self-fertile isolates are 5.66 and 5.95, respectively. Myxamoebae, however, are quite dissimilar in their nuclear DNA content. Those of the heterothallic isolates, Honduran 1–2 (A1) and Panamanian 2–4 (A7), have mean values of 3.81 and 3.69, whereas myxamoebae of the self-fertile Philippine-1 isolate were found to have a mean value of 6.07. Myxamoebae of the Ph-1 isolate are, therefore, at the same ploidy level as the Ph-1 Plasmodium. Mean DNA values for Ph-1 sporangial nuclei were in category 4C. Measurement of the DNA content of mitotic metaphases in sporangia at T = 6 hr confirmed that the mean DNA content of both Ph-1 myxamoebae and plasmodial nuclei is equivalent to 4C. It is concluded that nuclear phase alternance is lacking in the Ph-1 isolate and that the Plasmodium of this isolate develops by apogamy. Citing Literature Volume61, Issue4April 1974Pages 400-404 RelatedInformation