Portal de Periódicos da CAPES

Design of a Molecular Beacon DNA Probe with Two Fluorophores

2001; Wiley; Volume: 113; Issue: 2 Linguagem: Inglês

10.1002/1521-3757(20010119)113

1521-3757

Peng Zhang, Terry Beck, Weihong Tan,

RNA and protein synthesis mechanisms

Angewandte ChemieVolume 113, Issue 2 p. 416-419 Zuschrift Design of a Molecular Beacon DNA Probe with Two Fluorophores Peng Zhang, Peng Zhang Department of Chemistry and UF Brain Institute University of Florida Gainesville, FL 32601 (USA) Fax: (+1) 352-846-2410Search for more papers by this authorTerry Beck, Terry Beck TriLink BioTechnologies, Inc. 6310 Nancy Ridge Drive, Suite 101 San Diego, CA 92121 (USA)Search for more papers by this authorWeihong Tan Dr., Weihong Tan Dr. tan@chem.ufl.edu Department of Chemistry and UF Brain Institute University of Florida Gainesville, FL 32601 (USA) Fax: (+1) 352-846-2410Search for more papers by this author Peng Zhang, Peng Zhang Department of Chemistry and UF Brain Institute University of Florida Gainesville, FL 32601 (USA) Fax: (+1) 352-846-2410Search for more papers by this authorTerry Beck, Terry Beck TriLink BioTechnologies, Inc. 6310 Nancy Ridge Drive, Suite 101 San Diego, CA 92121 (USA)Search for more papers by this authorWeihong Tan Dr., Weihong Tan Dr. tan@chem.ufl.edu Department of Chemistry and UF Brain Institute University of Florida Gainesville, FL 32601 (USA) Fax: (+1) 352-846-2410Search for more papers by this author First published: 19 January 2001 https://doi.org/10.1002/1521-3757(20010119)113:2 3.0.CO;2-NCitations: 36 This work was partially supported by the Office of Naval Research Young Investigator Award N00014-98-1-0621 and by an NSF Career Award (CHE-9733650). We would like to thank Dr. Richard Hogrefe, Paul Imperial, Kelly Christianson, and the Trilink Oligonucleotide Synthesis group for producing the molecular beacons used in this study. Read the full textAboutPDF ToolsRequest permissionAdd to favorites ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Ein "molekulares Leuchtfeuer", ein DNA-Molekül mit einer Haarnadelschleife, wurde mit zwei Fluorophoreinheiten (F1 und F2) an den Enden ausgestattet, zwischen denen ein Fluoreszenz-Resonanzenergie-Transfer stattfindet. Dies ermöglicht die Verwendung für quantitative DNA- und RNA-Untersuchungen (siehe Schema). DNA-Konzentrationen bis herab zu 1.7×10−10 M konnten mit einem handelsüblichen Spektrometer mit Cumarin und 6-Carboxyfluorescein als Fluorophoreinheiten detektiert werden. Die Messung des Förster-Abstands für das Donor-Acceptor-Paar ist mit diesen DNA-Sonden ebenfalls möglich. Citing Literature Volume113, Issue2January 19, 2001Pages 416-419 This is the German version of Angewandte Chemie. Note for articles published since 1962: Do not cite this version alone. Take me to the International Edition version with citable page numbers, DOI, and citation export. We apologize for the inconvenience. RelatedInformation