Voltar
Artigo Acesso aberto Revisado por pares
BIBTEX RIS

Short-term, high-efficiency expression of transfected DNA.

1984; Taylor & Francis; Volume: 4; Issue: 8 Linguagem: Inglês

10.1128/mcb.4.8.1641

ISSN

1098-5549

Autores

D J Sussman, G Milman,

Tópico(s)

Animal Genetics and Reproduction

Resumo

We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.

Referência(s)