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19 EVALUATION OF VIABILITY AND PROLIFERATIVE ACTIVITY OF HUMAN UROTHELIAL CELLS CULTURED ONTO XENOGENIC TISSUE-ENGINEERED EXTRACELLULAR MATRICES

2011; Lippincott Williams & Wilkins; Volume: 185; Issue: 4S Linguagem: Inglês

10.1016/j.juro.2011.02.080

1527-3792

Niall F. Davis, Anthony Callanan, Barry B. McGuire, Hugh D. Flood, Tim M. McGloughlin,

Urological Disorders and Treatments

You have accessJournal of UrologyTrauma/Reconstruction: Trauma & Reconstructive Surgery1 Apr 201119 EVALUATION OF VIABILITY AND PROLIFERATIVE ACTIVITY OF HUMAN UROTHELIAL CELLS CULTURED ONTO XENOGENIC TISSUE-ENGINEERED EXTRACELLULAR MATRICES Niall Davis, Anthony Callanan, Barry McGuire, Hugh Flood, and Tim McGloughlin Niall DavisNiall Davis Limerick, Ireland More articles by this author , Anthony CallananAnthony Callanan Limerick, Ireland More articles by this author , Barry McGuireBarry McGuire Limerick, Ireland More articles by this author , Hugh FloodHugh Flood Limerick, Ireland More articles by this author , and Tim McGloughlinTim McGloughlin Limerick, Ireland More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.080AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The field of reconstructive urology has been influenced by tissue-engineered extracellular matrix (ECM) scaffolds over the last decade. However, contrasting techniques adopted by different research groups during manufacturing and preparation processes has led to apprehension among urologists when ECMs are considered for regenerative purposes in human patients. We aimed to assess whether ECM scaffold type, preparation technique applied and variable in vitro growth conditions significantly affect the ability of ECMs scaffolds to induce effective regeneration of the human genitourinary tract. METHODS Human urothelial cells (HUCs) were obtained by bladder biopsy and cultured onto the luminal and abluminal surfaces of decellularised porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM). In addition, HUCs were cultured in optimal in vitro growth conditions and in their expected in vivo urine environment. Attachment, viability and proliferative activity of HUCs were evaluated and compared by quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. RESULTS Luminal and abluminal surfaces of UBM demonstrated significantly greater HUC viability and proliferative activity compared to luminal and abluminal surfaces of SIS grafts (p<0.0001, fig 1). Culture of HUCs in a simulated in vivo urine environment significantly affected cell viability (p<0.0001). Proliferative activity was immeasurable on cell-seeded scaffolds that were cultured in a urine environment after 48hours of growth (p<0.0001). CONCLUSIONS This is the first comparative analysis of UBM and SIS. UBM demonstrates significantly greater regenerative potential for HUC's compared to SIS. However, the perceived potential for ECMs in reconstructive urology may be limited by their inability to induce effective urothelial regeneration in a urine environment. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e7 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.Metrics Author Information Niall Davis Limerick, Ireland More articles by this author Anthony Callanan Limerick, Ireland More articles by this author Barry McGuire Limerick, Ireland More articles by this author Hugh Flood Limerick, Ireland More articles by this author Tim McGloughlin Limerick, Ireland More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...