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ABO-Incompatible Renal Transplantation Without Antibody Removal Using Conventional Immunosuppression Alone

2015; Elsevier BV; Volume: 15; Issue: 6 Linguagem: Inglês

10.1111/ajt.13255

1600-6143

Nithya Krishnan, R. Coates, Sunil Daga, V. Carter, David Talbot, David Briggs, Robert Higgins,

Renal Diseases and Glomerulopathies

American Journal of TransplantationVolume 15, Issue 6 p. 1728-1729 Letter to the EditorFree Access ABO-Incompatible Renal Transplantation Without Antibody Removal Using Conventional Immunosuppression Alone N. Krishnan, Corresponding Author N. Krishnan Renal Unit, University Hospitals Coventry and Warwickshire, UK Corresponding author: Nithya Krishnan, Krishnan@uhcw.nhs.ukSearch for more papers by this authorR. Coates, R. Coates Renal Unit, The Newcastle upon Tyne Hospitals, UKSearch for more papers by this authorS. Daga, S. Daga Renal Unit, University Hospitals Coventry and Warwickshire, UKSearch for more papers by this authorV. Carter, V. Carter Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Newcastle, UKSearch for more papers by this authorD. Talbot, D. Talbot Renal Unit, The Newcastle upon Tyne Hospitals, UKSearch for more papers by this authorD. Briggs, D. Briggs Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Birmingham, UKSearch for more papers by this authorR. Higgins, R. Higgins Renal Unit, University Hospitals Coventry and Warwickshire, UKSearch for more papers by this author N. Krishnan, Corresponding Author N. Krishnan Renal Unit, University Hospitals Coventry and Warwickshire, UK Corresponding author: Nithya Krishnan, Krishnan@uhcw.nhs.ukSearch for more papers by this authorR. Coates, R. Coates Renal Unit, The Newcastle upon Tyne Hospitals, UKSearch for more papers by this authorS. Daga, S. Daga Renal Unit, University Hospitals Coventry and Warwickshire, UKSearch for more papers by this authorV. Carter, V. Carter Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Newcastle, UKSearch for more papers by this authorD. Talbot, D. Talbot Renal Unit, The Newcastle upon Tyne Hospitals, UKSearch for more papers by this authorD. Briggs, D. Briggs Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Birmingham, UKSearch for more papers by this authorR. Higgins, R. Higgins Renal Unit, University Hospitals Coventry and Warwickshire, UKSearch for more papers by this author First published: 24 April 2015 https://doi.org/10.1111/ajt.13255Citations: 7 See also: S Cohney et al and D Focosi AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat To the Editor: Masterson et al had shown that in their series of 20 patients with low blood group antibody titers (ABGAb) who underwent ABO-incompatible (ABOi) live donor renal transplantation with standard immunosuppression and without antibody removal had 100% patient and graft survival at the end of 36 months 1. We report two patients from our centers who received ABOi live donor renal transplantation with low-level ABGAb with standard immunosuppression and no antibody removal, but with opposite results. A 73-year-old female, blood group O, previous bone marrow transplantation with splenic radiation for leukemia, received a blood-group B kidney. Pretransplant anti-B IgG titer was 0 and IgM was 2 with low levels confirmed on several occasions pretransplant and immediately pretransplant. IgM titers were measured by direct agglutination, IgG titers by indirect agglutination with dithiothrietol, both using a gelcard method (Bio-Rad, Hemel Hempstead, UK). No donor HLA-specific antibodies were detected. Immunosuppression was basiliximab, tacrolimus, prednisolone, and mycophenolate. Posttransplant, the serum creatinine, was 65 umol/L on day 5. On the sixth day posttransplant, she was noted to have raised liver function tests. This was thought to be due to mycophenolate toxicity and hence mycophenolate was stopped. On day 8, her urine output had reduced significantly, creatinine had increased to 213, blood and urine cultures grew E. coli and anti-B titers were IgG 2 and IgM 8. She was started on anti-thymocyte globulin. The next day, the urine output reduced further and the anti-B titers were IgG 8 and IgM 64. A biopsy showed infarction of the kidney with acute antibody-mediated rejection (AMR) and she had transplant nephrectomy. The peak anti-B titers on day 13 were IgG 32 and IgM 2048. A 32-year-old female, blood group A, with renal failure due to diabetic nephropathy, received a kidney from her blood group AB mother. She had no anti-HLA donor-specific antibodies. The recipient's anti-B IgG titer was 1 and IgM was 8 with low levels confirmed immediately pretransplant. She received alemtuzumab induction and tacrolimus. After initial graft function, she was noted on day 3 to have red cell fragmentation with low platelets and raised LDH levels. She was thought to have microangiopathic hemolytic anemia (MAHA) and hence tacrolimus was stopped temporarily. On day 7, the serum creatinine rose further, the anti-B titers were IgG 8 and IgM 32, and HLA antibody screening was negative, biopsy showed thrombosis of the graft with AMR and the graft was removed the next day. Anti-B titers were IgG 32 and IgM 512 on day 8. In both these cases, the pretransplant blood group antibody levels were low, and there were no anti-HLA antibodies detected. We did not test for anti-angiotensin 1 receptor antibodies. Biopsy appearances confirmed acute AMR, and the rises in ABO antibody levels implicated these as the driver of graft failure. It can be speculated that an infective episode and drug toxicity or slight reduction in immunosuppression could potentially allow immunological escape. It is known that there is significant interinstitutional variation in the measurement of blood group antibodies by haemagglutination methods 2. We therefore developed a flow cytometry technique and demonstrated that significant anti-IgG response occurs only in O recipients with A1 or B donors 3. As only 3 of their 20 cases in the Masterton et al series fell into this group it is important to rule out any bias in antibody measurements before prescribing ABOi transplantation with conventional immunosuppression for all patients with low ABGAb. Though outcomes of ABOi transplantation are good irrespective of the titers 4, 5, our cases demonstrate that graft loss post-ABOi transplantation is not necessarily related to high pretransplant ABGAb. Thus, though the authors show excellent results, there may be failures as their numbers increase. N. Krishnan1,*, R. Coates2, S. Daga1, V. Carter3, D. Talbot2, D. Briggs4 and R. Higgins1 1Renal Unit, University Hospitals Coventry and Warwickshire, UK 2Renal Unit, The Newcastle upon Tyne Hospitals, UK 3Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Newcastle, UK 4Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Birmingham, UK *Corresponding author: Nithya Krishnan, Krishnan@uhcw.nhs.uk Disclosure The authors of this manuscript have no conflicts of interest to disclose as described by the American Journal of Transplantation. References 1 Masterson R, Hughes P, Walker RG, et al. ABO incompatible renal transplantation without antibody removal using conventional immunosuppression alone. Am J Transplant 2014; 14: 2807– 2813. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 2 Tyden G, Donauer J, Wadstrom J, et al. Implementation of a protocol for ABO-incompatible kidney transplantation–a three-center experience with 60 consecutive transplantations. Transplantation 2007; 83: 1153– 1155. CrossrefPubMedWeb of Science®Google Scholar 3 Krishnan NS, Fleetwood P, Higgins RM, et al. Application of flow cytometry to monitor antibody levels in ABO incompatible kidney transplantation. Transplantation 2008; 86: 474– 477. CrossrefCASPubMedWeb of Science®Google Scholar 4 Montgomery JR, Berger JC, Warren DS, et al. Outcomes of ABO-incompatible kidney transplantation in the United States. Transplantation 2012; 93: 603– 609. CrossrefCASPubMedWeb of Science®Google Scholar 5 Tanabe K, Ishida H, Inui M, et al. ABO-incompatible kidney transplantation: Long-term outcomes. Clin Transpl 2013; 307– 312. PubMedGoogle Scholar Citing Literature Volume15, Issue6June 2015Pages 1728-1729 AST and ASTS members - please log in via your Society website for full journal access.AST Members >> ASTS Members >> ReferencesRelatedInformation