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A Droplet‐Based, Composite PDMS/Glass Capillary Microfluidic System for Evaluating Protein Crystallization Conditions by Microbatch and Vapor‐Diffusion Methods with On‐Chip X‐Ray Diffraction

2004; Wiley; Volume: 116; Issue: 19 Linguagem: Inglês

10.1002/ange.200453974

1521-3757

Bo Zheng, Joshua D. Tice, L. Spencer Roach, Rustem F. Ismagilov,

Microfluidic and Capillary Electrophoresis Applications

Angewandte ChemieVolume 116, Issue 19 p. 2562-2565 Zuschrift A Droplet-Based, Composite PDMS/Glass Capillary Microfluidic System for Evaluating Protein Crystallization Conditions by Microbatch and Vapor-Diffusion Methods with On-Chip X-Ray Diffraction† Bo Zheng Dr., Bo Zheng Dr. Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorJoshua D. Tice, Joshua D. Tice Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorL. Spencer Roach, L. Spencer Roach Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorRustem F. Ismagilov Prof., Rustem F. Ismagilov Prof. r-ismagilov@uchicago.edu Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this author Bo Zheng Dr., Bo Zheng Dr. Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorJoshua D. Tice, Joshua D. Tice Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorL. Spencer Roach, L. Spencer Roach Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this authorRustem F. Ismagilov Prof., Rustem F. Ismagilov Prof. r-ismagilov@uchicago.edu Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805Search for more papers by this author First published: 28 April 2004 https://doi.org/10.1002/ange.200453974Citations: 29 † This work was supported by the NIH (R01 EB001903), and by Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by NSF. L.S.R. is supported by the Burroughs Wellcome Fund Cross-Disciplinary Training Program in Biophysical Dynamics. We thank Adam Lyon for preliminary experiments. We thank Phoebe Rice, Keith Moffatt, Andrzej Joachimiak, Chuan He, and Erica Duguid for valuable discussions and help with protein crystallography. Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under Contract No. W-31-109-Eng-38. Use of the BioCARS Sector 14 was supported by the National Institutes of Health, National Center for Research Resources, under grant number RR07707. Read the full textAboutPDF ToolsRequest permissionAdd to favorites ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Aus einem Tröpfchen: Proteine können mit Mikrobatch- und Dampfdiffusionstechniken in PDMS/Glas-Kompositbauteilen aus Volumina von wenigen Nanolitern kristallisiert werden (PDMS=Polydimethylsiloxan). Die Qualität der Kristalle kann direkt mithilfe von On-Chip-Röntgenbeugung beurteilt werden (siehe Bild). Citing Literature Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2004/z53974_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume116, Issue19May 3, 2004Pages 2562-2565 This is the German version of Angewandte Chemie. Note for articles published since 1962: Do not cite this version alone. Take me to the International Edition version with citable page numbers, DOI, and citation export. We apologize for the inconvenience. RelatedInformation