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Effect of TNF-α, IL-β, IL4 and TGF-β cytokines by Tuberculosis Systemic on blood-brain barrier and Neural disorders in Murine Experimental Model "

2015; Frontiers Media; Volume: 6; Linguagem: Inglês

10.3389/conf.fimmu.2015.05.00154

1664-3224

Salinas Lara Citlaltepetl, Cartillon Benavides Karen, Alvarado Híjar Wendy, Sánchez Garibay Carlos, Castillon Benavides Omar, Alejandro Guizar Miguel, Flores Barrada Manuel, Soto José, Campa Arvizu Paloma, Tena Suck Martha, Hernandez Pando Rogelio, Hernández Campos María, Jimenez Zamudio Luis, Rembao Bojórquez Daniel, Montaño Rendón Fernando,

Inflammation biomarkers and pathways

Event Abstract Back to Event Effect of TNF-α, IL-β, IL4 and TGF-β cytokines by Tuberculosis Systemic on blood-brain barrier and Neural disorders in Murine Experimental Model " Citlaltepetl Salinas Lara1*, Karen Cartillon Benavides2, Wendy A. Alvarado Híjar1, Carlos Sánchez Garibay1, Omar J. Castillon Benavides3, Miguel M. Alejandro Guizar4, Manuel A. Flores Barrada4, José L. Soto5, Paloma Campa Arvizu1, Martha L. Tena Suck1, Rogelio Hernandez Pando6, María E. Hernández Campos7, Luis A. Jimenez Zamudio8, Daniel Rembao Bojórquez1 and Fernando Montaño Rendón1 1 Instituto Nacional de Neurología y Neurocirugía, Mexico 2 Hospital Materno Infantil DEL ISSEMYM, Mexico 3 Hospital Regional 1o. de Octubre, Mexico 4 Universisdad Juarez Autonoma de Tabasco, Mexico 5 INSTITUTO NACIONAL DE NEUROLOGÍA Y NEUROCIRUGÍA, Neuroinfectología, Mexico 6 INSTITUTO NACIONAL DE CIENCIAS MÉDICAS Y NUTRICIÓN SALVADOR ZUBIRÁN, Patología Experimental, Mexico 7 Escuela Superior de Medicina del Instituto Politecnico Nacional, Maestría en Ciencias de la Salud y Doctorado en investigación en Medicina, Mexico 8 Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Naciona, Posgrado en Ciencias Químico Biológicas., Mexico Introduction: The mechanisms by which M. tuberculosis damages the central nervous system (CNF) and produces changes in the blood-brain-barrier (BBB) and/or in the blood-CSF barrier is not well understood yet. These changes could be dependent on the specific strain as well as on the disease´s progression time. Material and methods: In this study a strain of M. tuberculosis (LAM3) was isolated from a patient with meningitis, subsequently, BALB/c male mice were infected though the trachea with H37Rv and LAM3 strains. These mice were later on sacrificed at different days following infection (1, 3, 7, 14, 21, 28, 60, 120). Mice brains were obtained and stained with hematoxylin and eosin, argentic stain method of del Rio-Hortega, electron microscopy was used for BBB and CFS-BB, Ziehl-Neelsen and PCR and immunohistochemestry was used for citoquine detection (IL1-β, TNF-α, IFN-γ, TGF-β, IL-4). Results: Neuronal alterations were observed in the frontal cortex and the hippocampus with H37Rv and LAM3 (cell death, disorganization and eosinophilic degeneration). There were no CFU growth in the brain for neither strain, though PCR was positive for LAM3. The citoquines were positive in ependymal cells, endothelial cells and in choroid plexuses for both strains: IL-1β, TNF-α, IL-4 y TGF-B. LAM3 expressed IFN-γ with greater intensity. Structural abnormalities (cytoplasmic vacuolation, intercellular separation and necrosis) were shown at the Blood-CSF barrier for both LAM3 and H37Rv. Conclusion: Concluding by these findings that neuronal abnormalities in the frontal cortex, the hippocampus and the Blood-CSF barrier are present in post-infection with M tuberculosis, these findings being more severe in the LAM3 strain than in H37Rv and possibly mediated by citoquine presence. Keywords: mouse models, TNF-α, IL-β, Tuberculosis Systemic, blood-brain barrier disruption Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Infectious and parasitic diseases Citation: Salinas Lara C, Cartillon Benavides K, Alvarado Híjar WA, Sánchez Garibay C, Castillon Benavides OJ, Alejandro Guizar MM, Flores Barrada MA, Soto JL, Campa Arvizu P, Tena Suck ML, Hernandez Pando R, Hernández Campos ME, Jimenez Zamudio LA, Rembao Bojórquez D and Montaño Rendón F (2015). Effect of TNF-α, IL-β, IL4 and TGF-β cytokines by Tuberculosis Systemic on blood-brain barrier and Neural disorders in Murine Experimental Model ". Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00154 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 May 2015; Published Online: 14 Sep 2015. * Correspondence: Dr. Citlaltepetl Salinas Lara, Instituto Nacional de Neurología y Neurocirugía, México, 14269, Mexico, cisala69@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Citlaltepetl Salinas Lara Karen Cartillon Benavides Wendy A Alvarado Híjar Carlos Sánchez Garibay Omar J Castillon Benavides Miguel M Alejandro Guizar Manuel A Flores Barrada José L Soto Paloma Campa Arvizu Martha L Tena Suck Rogelio Hernandez Pando María E Hernández Campos Luis A Jimenez Zamudio Daniel Rembao Bojórquez Fernando Montaño Rendón Google Citlaltepetl Salinas Lara Karen Cartillon Benavides Wendy A Alvarado Híjar Carlos Sánchez Garibay Omar J Castillon Benavides Miguel M Alejandro Guizar Manuel A Flores Barrada José L Soto Paloma Campa Arvizu Martha L Tena Suck Rogelio Hernandez Pando María E Hernández Campos Luis A Jimenez Zamudio Daniel Rembao Bojórquez Fernando Montaño Rendón Google Scholar Citlaltepetl Salinas Lara Karen Cartillon Benavides Wendy A Alvarado Híjar Carlos Sánchez Garibay Omar J Castillon Benavides Miguel M Alejandro Guizar Manuel A Flores Barrada José L Soto Paloma Campa Arvizu Martha L Tena Suck Rogelio Hernandez Pando María E Hernández Campos Luis A Jimenez Zamudio Daniel Rembao Bojórquez Fernando Montaño Rendón PubMed Citlaltepetl Salinas Lara Karen Cartillon Benavides Wendy A Alvarado Híjar Carlos Sánchez Garibay Omar J Castillon Benavides Miguel M Alejandro Guizar Manuel A Flores Barrada José L Soto Paloma Campa Arvizu Martha L Tena Suck Rogelio Hernandez Pando María E Hernández Campos Luis A Jimenez Zamudio Daniel Rembao Bojórquez Fernando Montaño Rendón Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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