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A 460-kb DNA Sequence of the Escherichia coli K-12 Genome Corresponding to the 40.1-50.0 min Region on the Linkage Map

1996; University of Oxford; Volume: 3; Issue: 6 Linguagem: Inglês

10.1093/dnares/3.6.379

1756-1663

Takeshi Itoh,

Genomics and Phylogenetic Studies

The 465,813 base pair sequence corresponding to the 40.1–50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2–46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified anew asn-tRNA gene, designated asnW , between the asnT and asnU genes and a new lysogenic phage attachment site as the cis -element.