A method for the paper-chromatographic separation and identification of phenol derivatives, mould metabolites and related compounds of biochemical interest, using a “reference system”
1959; Elsevier BV; Volume: 1; Linguagem: Inglês
10.1016/0009-5907(59)80005-3
ISSN1878-1438
Autores Tópico(s)Dye analysis and toxicity
ResumoA paper-chromatographic procedure is described for the identification of phe metabolic products from moulds, especially from Penicillia species, and aliphatic compounds of biochemical interest. Information is given concerning the application of the method to the characterization of unknown substances encountered in various biological investigations, mg quantities of material being sufficient. The Rf values in six different solvent systems together with the corresponding colour reactions produced by ten standard reagents have been collected and presented in 21 Tables. In all, approx. 450 compounds were investigated. Original recordings were made on standard cards, which made it possible to present the Rf values as diagrams and to give the code of colour reactions for each compound separately. A standard numerical colour index was used for these colour recordings. The possible application of this procedure, within the biochemical, microbiological, medical and organic-chemical fields, to preliminary investigations of unknown mixtures is indicated. A special set-up was constructed for rapid analysis by means of which spraying as well as evaluation of six chromatograms could be carried out simultaneously. Two new stable diazonium reagents, o-dianisidine diazotate and 4-benzoylamino-2,5-dimethoxy-aniline, were found to be useful for detecting phenolic compounds and aliphatic keto acids. Several other reagents were tested with regard to their usefulness for spraying purposes in paper-chromatographic analysis.
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